Jan 16, 2020 · Unlike Tsix, LinxP is well conserved across mammals, suggesting an ancestral mechanism for random monoallelic Xist regulation. When introduced in the same TAD as Xist, LinxP switches from a silencer to an enhancer.

The recent discovery that LinxP (the promoter of the Linx noncoding RNA), which lies in the Tsix -TAD, acts as a long-range silencer of Xist across the Xist / Tsix -TADs boundary (Fig. 3 A2), suggests that inter-TAD cis -regulation is nonetheless possible—and functionally important—at the Xic (Galupa et al. 2020).

LinxP’s repressive action occurs at a distance of u0001170 kb and across a TAD boundary. Consistent with this action on Xist, LinxP binds two known repressors of Xist, the pluripotency factors Nanog and Oct4. How these factors repress Xist has remained unclear (reviewed in Minkovsky et al., 2012).

Dec 19, 2013 · Here we generate high-resolution maps of Xist binding on the X chromosome across a developmental time course using CHART-seq. In female cells undergoing XCI de novo, Xist follows a two-step mechanism, initially targeting gene-rich islands before spreading to intervening gene-poor domains.

Recently it was reported that the promoter region of the noncoding RNA Linx (LinxP) acts as a cis-regulatory element and long-range silencer to influence the choice of X chromosome to be inactivated independent of its transcriptional activity (49).

The X inactivation center (Xic is essential for induction of X chromosome inactivation and harbors two topologically associated domains (TADs) to regulate monoallelic Xist expression: one at the noncoding Xist gene and its upstream region, and the other at …

Apr 1, 1999 · We found evidence for an RNA antisense to Xist when examining Xist expression in embryonic stem (ES) cells using strand-specific probes by RNA fluorescence in situ hybridization (FISH).

Nov 1, 2011 · Random X chromosome inactivation (XCI), the eutherian mechanism of X-linked gene dosage compensation, is controlled by a cis -acting locus termed the X-inactivation center (Xic).

Nov 20, 2019 · Unlike Tsix, LinxP is well conserved across mammals, suggesting an ancestral mechanism for random monoallelic Xist regulation. When introduced in the same TAD as Xist, LinxP switches from a silencer to an enhancer.

Unlike Tsix, LinxP is well conserved across mammals, suggesting an ancestral mechanism for random monoallelic Xist regulation. When introduced in the same TAD as Xist, LinxP switches from a silencer to an enhancer.