MyoD is one of the earliest markers of myogenic commitment. MyoD is expressed at extremely low and essentially undetectable levels in quiescent satellite cells, but expression of MyoD is activated in response to exercise or muscle tissue damage.

Myod encodes a basic helix-loop-helix transcription factor belonging to a larger family of myogenic regulatory factors (MRFs) that together control determination and differentiation of all skeletal muscle cells.

Herein, we report a MyoD knock-in (MyoD-KI) reporter mouse expressing tdTomato at the endogenous MyoD locus. Expression of tdTomato in MyoD-KI mice recapitulated the endogenous MyoD expression dynamics in vitro and during the early phase of regeneration in vivo.

MyoD is a muscle-specific member of the basic helix–loop–helix (bHLH) class of proteins, a large family of transcription factors recognizing short DNA sequences (CANNTG), termed E-box motifs, in the regulatory regions of target genes .

Discovery of the myogenic regulatory factor family of transcription factors MYF5, MYOD, Myogenin and MRF4 was a seminal step in understanding specification of the skeletal muscle lineage and control of myogenic differentiation during development.

More than 20 years ago, studies established that Myod expression is largely controlled by just two enhancer regions located within a region 24 kb upstream of the transcription start site in mammals, which regulate Myod expression in the embryo, fetus and adult.

MyoD is a key regulator of skeletal myogenesis that directs contractile protein synthesis, but whether this transcription factor also regulates skeletal muscle metabolism has not been explored.

Dec 11, 2013 · MYOD is expressed at an early stage of myogenic differentiation, which, in turn, induces expression of other myogenesis-related genes, such as myogenin. In physiological conditions, MYOD can be phosphorylated or dephosphorylated.

Mar 3, 2015 · Sequence analysis revealed an enrichment for PBX/MEIS motifs at the subset of MyoD-specific sites bound by the chimera, and point mutations that prevent MyoD interaction with PBX/MEIS converted the chimera to a pure neurogenic factor.

Draw a picture of the DNA for three genes: MyoD, Myogenin, and a muscle gene like Myosin. For each gene, label the transcription unit and the regulatory region (the promoter). Within the regulatory region, label an enhancer for each of the three genes.