May 5, 2014 · A phylogenetic comparison to the Arabidopsis thaliana NB-LRR complement verifies the high conservation of the more ancient CCRPW8-type NB-LRRs. Use of RenSeq on cDNA from uninfected and late blight-infected tomato leaves allows the avoidance of sequence analysis of non-expressed paralogues.

Jan 11, 2010 · In this paper, the full-length cDNA cloning of the entire Arabidopsis LRR-RLK subfamily genes is reported.

Available data show that leucine-rich repeat (LRR) proteins play a crucial role in protein-protein interactions, recognition processes or immune reactions in both invertebrates and vertebrates. In the present study, we cloned and characterized a LRR cDNA from the mud crab Scylla paramamosain (SpLRR) by using the RACE strategy.

May 1, 2009 · The present study reports the first cloning and characterization of the full-length cDNA of the NBS-LRR candidate disease resistant gene from sweetpotato. The cloning of SPR1 enables us to test whether it plays a potential role in controlling sweetpotato diseases by transferring the gene into plants in the future.

LRR (Leucine rich repeat) proteins are involved in various biological processes. RACE was used to extend the 5' and 3' unknown sequence of a cDNA-AFLP fragment from cotton ovule, which had sequence similarity to the Arabidopsis thaliana LRR resistance-like (LRR-RL) protein.

Dec 1, 2001 · We conducted the yeast two-hybrid screening to identify intracellular signaling molecules that associate with 4-1BB. A novel leucine-rich repeat (LRR)-containing protein, herein named LRR-1, was found to specifically interact with the cytoplasmic domain of 4-1BB.

Oct 1, 2013 · The nucleotide-binding site (NBS)-leucine-rich repeat (LRR) gene family accounts for the largest number of known disease resistance genes and is one of the largest gene families in plant genomes. In this study, resistance gene analogs (RGAs) were isolated from peanuts based on the NBS domain.

A novel leucine-rich repeat (LRR) cDNA has been cloned from hemocytes of the black tiger shrimp Penaeus monodon by 5' rapid amplification of cDNA ends. The full-length of P. monodon LRR (PmLRR) consisted of 2604 bp with a 1686-bp …

Use of RenSeq on cDNA from uninfected and late blight-infected tomato leaves allows the avoidance of sequence analysis of non-expressed paralogues. RenSeq is a promising method to facilitate analysis of plant resistance gene complements.

Jun 23, 2009 · This set contains Gateway entry clones consisting of the full-length coding region of an LRR RLK cDNA in the vector pDONR/zeo from Invitrogen and Gateway plant expression clones in the pB35GWF vector. Growth Requirement: grow in LB media at 37C overnight. Marker: Background: Col (Columbia)