C. Jeré (or Curtis Jeré, often seen without the accent) is a metalwork company known for wall sculptures and household accessories. Jeré works range from representational to highly abstract, and are made and marketed by the corporation Artisan House.

HrtR specifically binds a 15-nt palindromic sequence in the hrtRBA promoter region, which is needed for hrtRBA repression. HrtR-DNA binding is abolished by heme addition, which activates expression of the HrtB-HrtA (HrtBA) transporter in vitro and in vivo.

We show that HrtR expressed as a fusion protein is a heme-binding pro-tein. Heme iron interaction with HrtR is non-covalent, hexaco-ordinated, and involves two histidines, His-72 and His-149. HrtR specifically binds a 15-nt palindromic sequence in the hrtRBA promoter region, which is needed for hrtRBA repres-sion.

Here, we report that the transcriptional regulator heme-regulated transporter regulator (HrtR) senses and binds a heme molecule as its physiological effector to regulate the expression of the heme-efflux system responsible for heme homeostasis in L. lactis.

A series of engineered biosensors with varied sensitivity and threshold were obtained by semi-rational design with site saturated mutation of HrtR. The modified metabolite-binding affinity of HrtR was confirmed by heme titration and molecular dynamic simulation.

The heme-regulated transport regulator, HrtR, belonging to TetR family, is responsible for heme sensing and is adjacent to hrtAB [57,66] (Fig. 1B) ( Table 1).

HrtR binds heme at the histidine residues His72 and His149, resulting in a coil-to-helix transition of the α4 helix in the heme-sensing domain and structural changes in HrtR, further leading to...

Feb 1, 2012 · We show that HrtR expressed as a fusion protein is a heme-binding protein. Heme iron interaction with HrtR is non-covalent, hexacoordinated, and involves two histidines, His-72 and His-149. HrtR specifically binds a 15-nt palindromic sequence in the hrtRBA promoter region, which is needed for hrtRBA repression.

Role of histidines His-72 and His-149 in HrtR binding to heme and to DNA. A, UV-visible spectra of 30 M ferric heme (hemin) and MBP-HrtR WT, H72A/H149L, and H72A/H149L (HH). WT and mutant...

May 22, 2020 · The heme-responsive regulatory system was designed by incorporation of heme biosensor with CRISPRi regulation. We synthesized a hybrid promoter containing the DNA-binding site (hrtO) of HrtR, whereas HrtR expression was driven by a …