Dec 1, 2016 · Intrinsic signal optical imaging (ISI) is a rapid and noninvasive method for observing brain activity in vivo over a large area of the cortex. Here we describe our protocol for...

Sep 17, 2021 · Here, we provide a protocol for ISI through a cranial window with an access port to identify the area of the primary visual cortex (V1) in a head-fixed mouse, followed by targeted viral vector injection, which enables subsequent two-photon imaging of …

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Here, we provide a protocol for ISI through a cranial window with an access port to identify the area of the primary visual cortex (V1) in a head-fixed mouse, followed by targeted viral vector injection, which enables subsequent two-photon imaging of V1 layer 6 corticothalamic neurons.

Intrinsic signal optical imaging (ISI) is a rapid and noninvasive method for observing brain activity in vivo over a large area of the cortex. Here we describe our protocol for mapping retinotopy to identify mouse visual cortical areas using ISI.

Automated identification of mouse visual areas with intrinsic signal imaging. Code for intrinsic signal optical imaging acquisition, stimulus presentation, and mouse visual area segmentation. View the Project on GitHub SNLC/ISI. Download ZIP File; Download TAR Ball; View On GitHub; ISI

Code for ISI image acquisition, stimulus presentation, and mouse visual area segmentation. Imager & Stimulator Programs. Imager.zip contains files for image acquisition, written by Dario Ringach and Ian Nauhaus. Stimulator.zip contains files for running a …

A repository for acquisition and analysis of intrinsic signal for construction of mouse retinotopy. Advanced Contains packages for visual stimulus display, camera control, and frame acquisition with abiltity to change visual stimulus parameters.

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Feb 8, 2022 · In mice, large scale cortical neural activity evokes hemodynamic changes readily observable with intrinsic signal imaging (ISI). Pairing ISI with visual stimulation allows identification of...