Jun 14, 2022 · Repair of Cas9-induced double-stranded breaks results primarily in formation of small insertions and deletions (indels), but can also cause potentially harmful large deletions.

Mar 30, 2023 · Additional mutagenesis in the HNH domain of nCas9 (H840A) removed this ability and reduced nCas9 (H840A)-mediated indel generation in HEK293T cells. Digenome-seq …

Mar 14, 2019 · CRISPR (clustered regularly interspaced short palindromic repeats) gene editing uses the endonuclease Cas9 combined with a guide RNA (gRNA) designed to cleave a …

In this study we review the most commonly used indel detection methods and using a robust, sensitive, and cost efficient Indel Detection by Amplicon Analysis method, we have …

Jan 1, 2017 · Taken together, our results demonstrate that cellular repair of CRISPR/Cas9 induced DSBs in the human HEK293 cell line model, gives rise to indel profiles that are …

In this study, we developed a method called cancer-specific InDel attacker (CINDELA) that selectively kills cancer cells. CINDELA targets multiple InDel mutations produced during …

Jun 11, 2024 · Here we establish a model system for investigating allelic heterogeneity at the single-cell level and identify indel scarring from non-simultaneous nuclease activity at gRNA …

The RNA-guided endonuclease Cas9 is a versatile genome editing tool with a broad range of applications from therapeutics to functional annotation of genes. Cas9 creates double-strand …

Nov 10, 2020 · We highlight recent advances that enable accurate and sensitive quantification of indel events in cells regardless of their genome complexity, turning a complex pool of different …

Repair of Cas9-induced double-stranded breaks results primarily in formation of small insertions and deletions (indels), but can also cause potentially harmful large deletions. While …