Feb 12, 2025 · Re-expression of IFNLR1 on cancer cell lines was achieved using CRISPR activation (CRISPRa) followed by evaluating chemotherapy-induced apoptosis using flow cytometry assays. Results: STING was not broadly expressed across the NSCLC cell lines.

Mar 15, 2007 · Interferon lambda-1 (IFN- λ 1/IL-29) is a member of the Type-III interferon family, which contains three ligands: IFN- λ 1, 2 and 3. These three ligands use the same unique heterodimeric...

Nov 11, 2020 · Overall, there are 21 human IFNs, (16 type-I, 12 IFNαs, IFNβ, IFNϵ, IFNκ, and IFNω; 1 type-II, IFNγ; and 4 type-III, IFNλ1, IFNλ2, IFNλ3, and IFNλ4), that induce pleotropic cellular activities essential for innate and adaptive immune responses against virus …

Four subtypes of IFNλ have been identified in humans: IFNλ1 (IL-29), IFNλ2 (IL-28A), IFNλ3 (IL-28B) and IFNλ4.

Dec 1, 2011 · In this article, we define the c-REL/p65 NF-κB heterodimer and IRF-1 as key transcriptional activators and ZEB1, B lymphocyte-induced maturation protein 1, and the p50 NF-κB homodimer as key repressors of the IFN-λ1 gene. We further show that ZEB1 selectively regulates type III IFNs.

Nov 2, 2006 · In conclusion, we have shown that IFN- λ 1, a cytokine produced in response to viral infection, activates both monocytes and macrophages producing a restricted panel of cytokines and may...

We investigated control of IFNL1 expression in human colon epithelial cells. We used the TLR3 agonist poly I:C to drive expression of IFNL1 in SW480 cells, and small interfering RNA (siRNA) to knockdown target transcription factors. We identified ZEB1 and BLIMP-1 as transcription factors that strongly inhibited IFNL1 expression in SW480 cells.

Jan 1, 2014 · We investigated control of IFNL1 expression in human colon epithelial cells. We used the TLR3 agonist poly I:C to drive expression of IFNL1 in SW480 cells, and small interfering RNA (siRNA) to knockdown target transcription factors. We identified ZEB1 and BLIMP-1 as transcription factors that strongly inhibited IFNL1 expression in SW480 cells.

Dec 1, 2017 · Clinical research with pegylated IFN-λ1 indicated that IFN-λ1 can induce anti-viral activity comparable to that of IFN-α but without the undesirable side effects [7]. Recently, IFN-λ1 has also been used to treat human papilloma infection [8], asthma [9] and lung cancer [10].

Jul 26, 2024 · Here, we investigate the activity of lipidoid nanoparticle (LNP)-formulated mRNA encoding human IFNλ1 (ETH47), which is a critical driver of innate immunity at mucosal surfaces protecting from viral infections.