
Immunofluorescence Staining Using IBA1 and TMEM119 for
Oct 27, 2019 · This is a protocol for the dual visualization of microglia and infiltrating macrophages in mouse brain tissue. TMEM119 (which labels microglia selectively), when …
IBA1 Immunohistochemistry Staining Procedure - YouTube
This video covers the step by step protocol for IBA1 immunohistochemistry to target and visualize microglia. Demonstration by Fiona Ramnaraign. Video made by...
Different Methods for Evaluating Microglial Activation Using Anti ...
In a first step, we analyzed the progression of microglia activation in the medial corpus callosum using the (i) quantification of anti-ionized calcium-binding adaptor protein-1 (IBA1) + cell …
Standard Protocol for Immunohistochemistry of Microglia using Iba1 …
"Anti Iba1, Rabbit (for Immunocytochemistry)" is an excellent microglial marker antibody since it can stain even microglial processes. Here, we describes the protocol and points to note when …
Analyzing microglial phenotypes across neuropathologies: a …
Iba1 is a reliable cytoplasmic microglial marker with a strong signal, labelling both cell bodies and processes (Fig. 3, Step 1, Supplementary Fig. 2a). Nevertheless, there is a major pitfall in …
Iba1 immunoreactivity is enhanced following an antigen retrieval ...
We immunostained with anti-Iba1 antibody as our primary interest, but also stained some sections in parallel with 4G8 antibody for anti-Aβ staining comparison. Iba1 immunoreactivity was best …
Staining of HLA-DR, Iba1 and CD68 in human microglia reveals …
Aug 15, 2017 · Here, we directly compared the expression of HLA-DR, Iba1 and CD68 in microglia with different phenotypes, ranging from ramified to amoeboid, to foamy …
Immunofluorescence Staining Using IBA1 and TMEM119 for
Oct 27, 2019 · IBA1 is a calcium binding protein that provides exceptional visualization of microglial morphology, including fine distal processes, as confirmed by electron microscopy 6. …
IBA1 Marker: Myeloid Cell Identification and Neuroimmune Impact
Mar 14, 2025 · Immunohistochemical (IHC) staining for IBA1 is widely used to visualize myeloid cells in tissue sections. The process begins with tissue fixation, typically using …
I am staining mouse brain microglia with Iba1 and I am getting a …
Often the cause of uneven stairing is poor perfusion. The cortex is stained well, and striatum bad because striatum separated by white matter. PFA does not penetrate there during additional...