May 26, 2017 · Indirect immunofluorescence, or secondary immunofluorescence, is a technique used in laboratories to detect circulating autoantibodies in patient serum. It is used to diagnose autoimmune blistering diseases.

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The indirect immunofluorescent test has three elements to consider in the result: 1. Positive or negative fluorescence. A negative test is strong evidence against a diagnosis of SLE but not conclusive. 2. The titer (dilution) to which fluorescence remains positive (provides a reflection of the concentration or avidity of the antibody).

Liver-related autoantibodies are crucial for the correct diagnosis and classification of autoimmune liver diseases (AiLD), namely autoimmune hepatitis types 1 and 2 (AIH-1 and 2), primary biliary cirrhosis (PBC), and the sclerosing cholangitis variants in adults and children.

Indirect immunofluorescence (IIF) plays an important role in immunological and immunometric assays for detecting and measuring autoantibodies. This technology was the first multiplex method used to detect cardinal autoantibodies for the diagnosis of autoimmune diseases.

Feb 15, 1983 · The indirect immunofluorescence (IFL) test is the method of choice for the detection of new organ-specific autoantibodies. It is also the standard method of detection of antibodies to pancreatic islet cells, adrenal cortex, and …

Twenty-seven patients affected by ulcerative colitis or Crohn's disease were submitted to a secretin-cerulein test, oral glucose test (OGT) and to indirect immunofluorescence (IFL) for detection of autoantibodies against exocrine and endocrine tissue.

Apr 1, 2011 · In diagnostic practice, a negative test for SMA by IFL is in itself sufficient. But a positive test, especially if weak, should be followed up either by IFL on other tissues to ascertain the filament subspecificity, as described below, or a molecular assay using purified F-actin.

Jan 1, 2011 · The ANA test also known as immunofluorescence ANA (IF-ANA) or fluorescent (F-ANA) test is widely available and is based on the detection by IFL of antibodies directed against a variety of antigens localized to the cell nucleus [ 6, 7 ].

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