Half maximal inhibitory concentration (IC 50) is a measure of the potency of a substance in inhibiting a specific biological or biochemical function. IC 50 is a quantitative measure that indicates how much of a particular inhibitory substance (e.g. drug) is needed to inhibit, in vitro, a given biological process or biological component by 50%. [1]

The concentration that provokes a response halfway between the Blank and the NS value is sometimes called the absolute IC50, The horizontal dotted lines show how 100% and 0% are defined, which then defines 50%. This term is not entirely standard.

The IC 50 is the concentration of drug required for 50% inhibition. IC 50 is an operational term dependent on the assay conditions. IC 90 or IC 99 is sometimes used when complete inhibition is required.

Dec 31, 2019 · IC50 stands for inhibitory concentration 50%. That is, the concentration of inhibitor required to reduce the biological activity of interest to half of the uninhibited value. Because it does not directly measure a binding equilibrium, IC50 is less precise than Ki or Kd.

The half-maximal inhibitory concentration (IC 50) is the most widely used and informative measure of a drug’s efficacy. It indicates how much drug is needed to inhibit a biological process by half, thus providing a measure of potency of an antagonist drug in pharmacological research.

Since the concept of the half maximal inhibitory concentration (IC(50)) is used extensively for studying reversible inhibition enzymatic reactions, it is important to clearly understand the experimental design and the mathematical modeling techniques used to generate IC(50) values.

May 11, 2021 · The absolute IC50 is the concentration that produces a result halfway between the Blank and NS values. The horizontal dotted lines indicate how 100 percent and 0 percent are described, which then defines 50 percent. This is a word that isn’t widely used.

Oct 24, 2019 · IC 50 represents the concentration at which a substance exerts half of its maximal inhibitory effect. This value is typically used to characterize the effectiveness of an antagonist in inhibiting a specific biological or biochemical process (ex. phosphorylation).

The term IC 50 refers to the half maximal inhibitory concentration and is a highly relevant concept for drug discovery and analysis. A study with this might be trying to look at how concentrations of a drug change over time in a body and what interactions occur during that period; in clinical settings, these are called Drug-Drug Interaction ...

The concentration of a drug or inhibitor needed to inhibit a biological process or response by 50%. IC50 is commonly used as a measure of drug potency in whole cell assays. IC50 assays are also used for screening in target-based drug discovery campaigns.