May 30, 2023 · Here, we show that oscillatory Hes1 expression down-regulates the expression of the cyclin-dependent kinase inhibitor p21 (Cdkn1a), which delays cell-cycle progression, and thereby activates the proliferation of mouse neural stem cells (NSCs).

Sep 28, 2024 · The results confirmed the up-regulation of Wnt3 and β-Catenin, and suppression of Nocth1, Hes1 protein level after overexpression of p21 (Fig. 5D, OE-p21G2TKO).

HES1, a target of Notch signaling, controls pituitary cell division and inhibits expression of the cell cycle inhibitors p27, p57, and p21 during embryonic development. Properly timed and tightly regulated development of the pituitary gland is critical to …

Hes1 is one mammalian counterpart of the Hairy and Enhancer of split proteins that play a critical role in many physiological processes including cellular differentiation, cell cycle arrest, apoptosis and self-renewal ability.

Research indicates that oscillating expression of Hes1 suppresses p21, whereas continuous overexpression of Hes1 leads to upregulation of p21. As a result, Hes1 plays a critical role in controlling neural stem cell proliferation via p21 (Maeda et al., 2023).

Additionally, Hes1 mutant pituitaries have ectopic expression of p21 in Rathke's pouch progenitors, an area coincident with increased cell death. These observations taken together indicate a role for HES1 in the control of cell cycle exit and in mediating the balance between proliferation and differentiation, allowing for the properly timed ...

Aug 8, 2008 · Suppression of p21-initiated senescence by HES1 (A–C) Human fibroblasts (HFs) were transduced (day 0) with a p21 Cip1 expression cassette flanked by loxP sites (p21) or a loxp vector (vector).

High-level persistent expression of the HES1 transcriptional repressor, which oscillates with an ultradian periodicity in proliferative neural stem cells (NSCs), is thought to mediate quiescence. However, it is not known whether this is due to a change in levels or dynamics.

Jan 1, 2013 · Using real-time PCR, we examined expression of Hes1 and p21 in FACS-sorted HSCs and HPCs populations from leukemia and control mice. The results showed that Hes1 expression in the HSCs-enriched population was higher than that in HPCs-enriched population from both leukemia (p = 0.003) and control mice (p = 0.002).

We found that lentivector-mediated HES1 expression in CD34+ cells inhibited cell cycling in vitro and cell expansion in vivo, associated with upregulation of the cell cycle inhibitor p21 (cip1/Waf1) (p21). The HES1 DNA-binding domain was required for these actions.