HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic sulfonic acid buffering agent. It is one of the twenty Good's buffers.

Gibco HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. The addition of 10–25 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO 2 incubator.

2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid is a HEPES that is ethanesulfonic acid in which one of the methyl hydrogens is replaced by a 4-(2-hydroxyethyl)piperazin-1-yl group. A Good's buffer substance, pKa = 7.55 at 20 ℃. It is a HEPES and an organosulfonic acid.

HEPES, also known as 4-(2-Hydroxyethyl)piperazine-1-ethane-sulfonic acid, is a zwitterionic N-substituted aminosulfonic acid buffer. It is effective in the pH 6.8-8.2 range, with a pKa of 7.48 at 25°C.

HEPES is commonly used as a biological buffering agent in biochemical research that helps maintain a stable pH in aqueous solutions. It is effective as a buffer, especially in the pH range of 6.8 to 8.2.

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture.

HEPES（4-羟乙基哌嗪乙磺酸） 是一种常用的 两性离子缓冲液，在37°C下的pKa为7.3。 在培养基中我们除了 碳酸氢钠 外，还应使用HEPES，因为重要的是要在培养基中保持足够的碳酸氢盐以达到营养目的。 如果需要，HEPES可以以10mM至25mM的最终浓度添加到 细胞培养基 中，以提供额外的缓冲能力。 较低的浓度通常不足以控制pH的波动，而较高的浓度通常是有毒的。 由 …

HEPES, a nonvolatile zwitterionic chemical buffering agent, is broadly applied in cell culture. HEPES is effective at pH 6.8 to 8.2. HEPES is also a potent inducer of lysosome biogenesis.

HEPES buffered saline (HBS) is a formulation optimized for maintaining isotonicity and pH stability under physiological conditions. HEPES: Acts as the buffer to maintain pH, especially in the range of 6.8 to 8.2. NaCl (Sodium Chloride): Provides osmotic balance.

Apr 13, 2022 · HEPES, a zwitterionic buffer, maintains pH stability, influences cellular functions, and inhibits prion protein conversion, making it essential in biological and medical research.