Jun 1, 2017 · These results demonstrate that AG + HA + FGN microbeads support HUVEC sprouting both within and between adjacent microbeads, and can promote distributed vascularization of an external matrix. Such modular microtissues may have utility in treating ischemic tissue by rapidly re-establishing a microvascular network.

The microbead matrix consisted of an agarose (AG) base to prevent aggregation, combined with cell-adhesive components of fibrinogen (FGN) and/or hydroxyapatite (HA). Microbeads encapsulating a co-culture of human umbilical vein endothelial cells (HUVEC) and fibroblasts were prepared and characterized.

Fibrillary glomerulonephritis (FGN) is found in approximately 1% of native kidney biopsies and was traditionally defined by glomerular deposition of fibrils larger than amyloid (12–24 nm diameter) composed of polyclonal IgG.

Fibrillary glomerulonephritis (FGN) is a rare primary glomerular disease that commonly presents clinically with hypertension, proteinuria, microscopic hematuria, and varying degree of renal insufficiency.

May 12, 2020 · Fibrillary glomerulonephritis (FGN) is a rare glomerular disease that often progresses to kidney failure requiring kidney replacement therapy. We have recently identified a novel biomarker of FGN, DnaJ homolog subfamily B member 9 (DNAJB9).

Figure 5-12: Protein content of AG, AG+HA, AG+FGN, and AG+HA+FGN microbeads that had been pre-cultured for 1 and 7 days (0.5ml and 1ml). Scale bar: 100 µm. - "Developing Pre-Vascularized Modular Microenvironments for Ischemic Applications"

Mar 5, 2019 · FGN is a rare form of glomerulonephritis that was first described in 1977 1 and is defined by the ultrastructural finding of organized, randomly oriented, nonbranching fibrils with a mean diameter of 20 nm (range 15–25 nm).

Jun 13, 2013 · Glomerulonephritis (GN) of the renal allograft in the setting of HepC most commonly manifests as type 1 membranoproliferative GN (MPGN), either representing recurrence of the original disease or arising de novo.

Perhaps the most common form of these rare glomerulopathies, FGN is identified by smudgy glomerular staining for IgG by immunofluorescence and accumulation of randomly oriented, nonbranching fibrils with a diameter of 15 to 25 nm. 1,2 Although FGN is typically polyclonal, monoclonal forms of FGN have been described.

The incorporation of HA and FGN within the microbeads permitted cell adhesion and spreading within the otherwise non-adherent AG. By developing these AG+HA+FGN microbeads, we have increased microbead production yield, improved injectability, and achieved a more homogeneous vascular network.