This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with HA. HA in the sample or standard competes with a fixed amount of biotin-labeled HA for sites on a pre-coated antibody specific to HA. Free components are washed off.

SKU: UNFI0033 Product Type: ELISA Kit Size: 96 Assays Sensitivity: 0.938ng/ml Range: 1.563-100ng/ml ELISA Type: Competitive Synonyms: HA, Hyaluronic Acid Reactivity: Universal

The Hyaluronan Enzyme-Linked Immunosorbent Assay (HA ELISA), a competitive 96-well assay, offers a simple, effective method for determining Hyaluronic Acid (HA) levels in human and animal biological fluids or cell supernatants.

This Human Hyaluronic acid (HA) ELISA Kit employs a two-site sandwich ELISA to quantitate HA in samples. An antibody specific for HA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHA present is bound by the immobilized antibody.

The Hyaluronic Acid Sandwich ELISA is a 96-well, quantitative immunoassay designed for in vitro measurement of HA levels in cell culture supernatant, or human/animal biological fluids.

The microtiter plate provided in this kit has been pre-coated with an antibody specific to HA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to HA.

Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1,000×g at 2-8oC within 30 minutes of collection. Remove plasma and assay immediately or store samples in aliquot at -20oC or -80oC for later use. Avoid repeated freeze/thaw cycles.

Hyaluronic acid ELISA kits are assays designed to detect the glycosaminoglycan hyaluronic acid. Also known as hyaluronate, it is present in the connective tissue of almost all vertebrates. It plays an important role in the health of bones and cartilage.