iSPE-HILIC cartridges are designed for the purification of polar and hydrophilic compounds using Hydrophilic Interaction Liquid Chromatography (HILIC). The packed stationary phases are charge modulated hydroxyethyl amide silicas which are covalently bonded with neutral, positively charged, and negatively charged hydrophilic functional groups.

HILIC can be described as a variation of Reversed Phase Liquid Chromatography (RPLC) performed using a polar stationary phase. The mobile phase employed in HILIC is highly organic in nature (>60% solvent, typically acetonitrile) containing a small percentage of aqueous solvent/buffer or other polar solvent. The water in the mobile phase forms an

Publication outlines a HILIC-based clean‐up method enabling complete separation of polar lipids containing four fatty acyl residues from non-polar lipid classes using iSPE® HILIC columns.

GlycoWorks HILIC SPE offers a robust, reliable solution for cleanup and concentration of glycans from complex matrices. Its application during the sample preparation of 2-AB labeled glycans helps ensure that an analyst reliably obtains successful results when studying protein glycosylation. References

Here, we demonstrated the glycopeptides enrichment efficiency of iSPE®-HILIC material for glycoproteomics application using a well characterised glycoprotein standard.

Dec 21, 2016 · We can confidently attempt and optimize HILIC at the stage of SPE within a given method, where HILIC of extracts in the LC dimension may not work with adequate chromatographic reproducibility. This article is part of Robert MacNeill’s (Envigo) quarterly column for Bioanalysis Zone which focuses on quantitative method design.

Apr 1, 2024 · We developed a sensitive method for N-glycan characterization using procainamide (ProcA) labelling and online solid phase extraction (online SPE). N-glycans were enzymatically released, then labeled with ProcA and cleaned up via the online SPE using HILIC chemistry (online HILIC SPE).

HILICON offers novel UHPLC/HPLC columns and SPE cartridges/plates for analysis of polar compounds in HILIC (hydrophilic interaction liquid chromatography) mode.

Jun 1, 2014 · To solve this problem, hydrophilic interaction liquid chromatography-solid phase extraction (HILIC-SPE) directly combined with protein precipitation (PPT) was developed for the selective extraction of triptorelin from plasma. The extracts were analyzed by reversed-phase liquid chromatography (RPLC).

This application brief demonstrates the benefits of using HILIC as an ideal separation mode for glycans as well as the robustness of HILIC in an SPE format.