The GUS reporter system (GUS: β-glucuronidase) is a reporter gene system, particularly useful in plant molecular biology [1] and microbiology. [2] Several kinds of GUS reporter gene assay are …

The beta-glucuronidase (GUS) gene is used extensively in plant biology studies; this analysis summarizes its advantages and limitations. With the advances in genomic sequencing and …

GUS fusions are now widely used to study plant-pathogen and plant-symbiont interactions. They can be used both to study expression of particular genes and to mark and monitor populations …

What is virus associated DNA, and why do I have to order it? Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. …

In 1987, Richard Jefferson et al. (1) demonstrated the application of a new reporter gene system in transgenic plants. The reporter gene was the uidA gene of Escherichia coli that encodes the …

Gene fusions can be defined as DNA constructions (performed in vitro or in vivo) that result in the coding sequences from one gene (reporter) being transcribed and/or translated under the …

We have directed the evolution of a GUS variant that is significantly more resistant to both glutaraldehyde and formaldehyde than the wild-type enzyme. A variant with eight amino acid …

In 1987, Richard Jefferson et al. (1) demonstrated the application of a new reporter gene system in transgenic plants. The reporter gene was the uidA gene of Escherichia coli that encodes the …

Feb 27, 1995 · The pGUS1 and pGUS2 vectors provide a simple, reliable and extremely sensitive reporter-gene system that allows quantitative measurement of promoter activity of yeast DNA …

In this chapter, I review several useful features of the GUS gene that make it a superior reporter gene for plant studies, and describe detailed protocols for the assay of beta-glucuronidase in …