The Basics of Gateway cloning reactions. BP reaction—to create a Invitrogen Gateway entry clone; LR reaction—to create a Gateway expression clone; One tube format—to create a …

Since Gateway Cloning is moving elements from one plasmid to another, they do not refer to integration and excision, but rather the BP reaction (BP→ LR) and the LR reaction (LR→ BP). …

Gateway™ LR Clonase™ II enzyme mix catalyzes the in vitro recombination between an entry clone (containing a gene of interest flanked by att L sites) and a destination vector (containing …

If you are performing the BP or LR recombination reactions for the first time, we recommend that you follow the detailed protocols provided in the manual. Perform a BP recombination reaction …

Gateway® BP ClonaseTM II enzyme mix is a proprietary enzyme and buffer formulation containing the bacteriophage lambda recombination protein Integrase (Int), the E. coli …

Figure 2: The Gateway system adopts phage integration into the BP and LR reactions. The BP reaction creates an attL-flanked entry clone. The LR reaction creates an expression clone with …

The BP clonase enzyme mix recombines attB sites with attP sites, generating attL and attR sites; whereas the LR clonase enzyme mix catalyzes the reverse reaction (Fig. 1A). Each …

Gateway technology facilitates cloning of genes into and back out of multiple vectors via site-specific recombination. Once a gene is cloned into an Entry clone, you can then move the …

Single-step BP/LR Clonase reaction protocol. DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money. Reduce the overall …

Technology. If you are performing the BP or LR recombination reactions for the first time, we recommend that you follow the detailed protocols provided in the manual. BP Recombination …