Green fluorescent protein (GFP) is increasingly being used in plant biology from the cellular level to whole plant level. At the cellular level, GFP is being used as an in vivo reporter to assess frequency of transient and stable transformation.

For what applications is GFP used in plant research? GFP is increasingly being used as a universal reporter in plant biology from the cellular to whole plant level: in vivo reporter to assess frequency of transient and stable transformation; monitoring …

Jan 18, 2025 · Explore the techniques and diverse applications of GFP gene expression in molecular biology and imaging. Green fluorescent protein (GFP) has become a vital tool in biological research due to its ability to emit bright green fluorescence when exposed to light.

Nov 8, 2018 · Here, to overcome this limitation, we generated transgenic petunia plants expressing eYGFPuv, a CpYGFP derivative exhibiting bright fluorescence under invisible ultraviolet (UV) light excitation,...

Here, we report that an extensively modified GFP is a versatile and sensitive reporter in a variety of living plant cells and in transgenic plants.

The green fluorescent protein (GFP) is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range. [2][3] The label GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria and is sometimes called avGFP.

Jul 1, 2002 · This review describes exciting advances in the use of green fluorescent protein (GFP) and its spectral derivatives to conventional and new imaging techniques in plants. GFP can be used in the...

Green fluorescent protein (GFP) has emerged as a powerful new tool in plant biotechnology research and applic- a tions. The purpose of this review is to briefly chronicle GFP research in plants and to summarize some of the bold directions this relatively new tool is taking plant biotechnology. GFP was first cloned and sequenced

A synthetic green fluorescent protein (GFP) gene (pgfp) was constructed to improve GFP expression in plants. Corn and tobacco protoplast transient assays showed that pgfp gave about 20-fold brighter fluorescence than the wild-type gene (gfp).

In this chapter, we describe some of the pitfalls affecting gfp expression and detection in plants, and describe modified forms of the gene and new techniques which have helped to overcome these problems. Cryptic splicing of gfp mRNA in Arabidopsis.