May 12, 2021 · Despite introducing four mutations into the Enhancer to reduce affinity for GFP, specific interaction with analyte (Mdm2) could only be discerned in vitro using fluorescence melt curve analysis.

They discovered that FMRP regulates neural stem cells through controlling the expression of the E3 ubiquitin ligase MDM2. They found that treatment with an inhibitor of MDM2 called Nutlin-3 rebalanced neural stem cell activities and rescued cognitive …

Mar 19, 2020 · In the experiment with Mdm2 miRNA knockdown, a combination of mouse anti-GFP (Clontech, JL-8, 1:500), rabbit anti-Tom 20 (Santa Cruz Biotechnology; FL-145; 1:50 dilution), and chicken...

Jan 9, 2025 · Delays in mitosis trigger p53-dependent arrest in G1 of the next cell cycle, thus preventing repeated cycles of chromosome instability and aneuploidy. Here we show that MDM2, the p53 ubiquitin...

Jan 2, 2018 · MARCH 7 catalyzes Lys 63 ‐linked polyubiquitination of Mdm2, which impedes Mdm2 autoubiquitination and degradation, thereby leading to the stabilization of Mdm2. MARCH 7 also promotes Mdm2‐dependent polyubiquitination and degradation of p53.

Here we show that, following DNA damage, the F-box protein FBXO31, a potential tumor suppressor, is responsible for promoting MDM2 degradation and therefore is essential for the increase in p53 levels. The tumor suppressor p53 plays a …

MCF-7 breast cancer cells transfected with pCMV-GFP control (GFP-control), MDM2-FL (90 kDa), P2-MDM2-10 (87 kDa), and MDM2-Δ5 were analyzed 24 hours after transfection. GAPDH (36 kDa) was used as loading control. Primary antibodies were anti-MDM2 OP146 (Merck Millipore) and anti-GAPDH (Santa Cruz). Figure 4.

May 31, 2022 · Here, we show that MDM2 binds, inhibits, ubiquitinates, and destabilizes poly (ADP-ribose) polymerase 1 (PARP1). When cellular MDM2 levels are increased, this leads to accelerated progression of DNA replication forks, much like pharmacological inhibition of PARP1.

Mar 15, 2011 · In immunoprecipitation assays, MDM2 was coprecipitated with GFP-MIP, but not GFP-FLAG or GFP alone when expressed in human colon carcinoma HCT116-p53+/+ cells (Fig. 4A) expressing wild-type p53. We examined the ability of GFP-MIP to activate the p53 pathway.

We confirmed these results by observations of recruitment of GFP-tagged Mdm2 to mKate-Mito-labeled mitochondria in living cells (Fig. 5B). Thus, Mdm2 is recruited to mitochondria that have lost their membrane potential, and this relocation does not require the presence of parkin.