Deactylation by SIRT1 enables liquid–liquid phase separation of IRF3 ...
Jul 25, 2022 · By developing a genetic code expansion orthogonal system, we demonstrated the presence of an acetyl moiety at specific IRF3/IRF7 DBD site/s abolish IRF3/IRF7 LLPS and …
pTRIP-GFP-IRF3 - Addgene
Plasmid pTRIP-GFP-IRF3 from Dr. Nicolas Manel's lab contains the insert IRF3 and is published in Cell Rep. 2019 Feb 26;26(9):2377-2393.e13. doi: 10.1016/j.celrep.2019.01.105. This …
IFN-regulatory factor 3-dependent gene expression is defective in
Dec 16, 2003 · Embryonic fibroblasts from wild-type and Tbk1-deficient mice were cotransfected with a vector encoding an IRF3-GFP fusion protein, and nuclear translocation of IRF3 was …
USP22 promotes IRF3 nuclear translocation and antiviral responses …
Mar 4, 2020 · Here, we discovered that cytoplasmic USP22 promoted nuclear translocation of IRF3 by deubiquitianting and stabilizing KPNA2 after viral infection.
MAVS deSUMOylation by SENP1 inhibits its aggregation and
May 15, 2023 · Our findings implicate SUMOylation in MAVS phase separation and suggest a thus far unknown regulatory process by which IRF3 can be efficiently recruited and released to …
Activation of Interferon Regulatory Factor 3 in Response to …
We found that in response to several DNA-damaging agents, IRF3 is phosphorylated, binds to CBP, moves to the nucleus, and increases transcription from its binding sites. These data …
AXIN1 boosts antiviral response through IRF3 stabilization and …
Oct 9, 2024 · Here, we identify that AXIN1 acts as an effective regulator of antiviral innate immunity against both DNA and RNA virus infections. In the resting state, AXIN1 maintains the …
Condensation of cellular prion protein promotes renal ... - Science
2 days ago · In addition, purified recombinant GFP-PrP-K>A failed to form droplets in vitro (fig. S13, C and D), indicating that lysine residues are crucial for PrP C phase separation. ...
pTRIP-GFP-IRF3 Sequences (1) - Addgene
Learn about new materials in the repository and helpful community resources. Have questions about your order, deposit, or a material? Addgene is a nonprofit plasmid repository. We store …
RNF149 modulates the type I IFN innate antiviral immune …
2 days ago · (K) WT and IRF3-KO HEK293T cells transfected with vector or Myc-RNF149 plasmids for 36 h were infected with VSV-GFP for 12 h and the viral titer of VSV was detected …