Compared with GD2 levels in untreated SH-SY5Y cells, levels of GD2 in SH-SY5Y cells increased 2.5-fold in response to fenretinide under the same conditions used for the GD3 experiments (Fig. 5).

In summary, flow cytometric characterization of SH-SY5Y cells provides a first comprehensive overview of NB-associated CD surface molecule antigens, yielding ample options for pathology studies and potential future immunotherapeutic paradigms.

Sep 9, 2015 · We found a significant IFNγ release by GD2 CAR T cells over 48-hour cocultures with SH-SY5Y. In addition, we observed that GD2 CAR T cell action was associated with a dramatic release of a potent death ligand, namely TRAIL.

One possible explanation for our findings is that a subset of SY5Y cells may express a level of GD2 just below the threshold antigen density required to trigger killing by the “wild-type” GD2 CAR-engineered T cells. SY5Y exhibits a fairly broad expression pattern spanning greater than 2 logs as measured by flow cytometry (see Fig. 3D ...

MES cells are greatly enriched in post-therapy and relapsing tumors and are characterized by decreased expression of GD2. This event may cause failure of GD2-based immunotherapy. NK cells represent a key innate cell subset able to efficiently kill tumors.

GD3 and GD2 are two types of disialogangliosides (GDs) that include two sialic acid residues. The expression of GD3 and GD2 in various cancers is mostly upregulated and is involved in tumor...

In this study, we establish potent synergy for the combination of anti-GD2 and anti-CD47 in syngeneic and xenograft mouse models of neuroblastoma, where the combination eradicates tumors, as well as osteosarcoma and small-cell lung cancer, where the combination significantly reduces tumor burden and extends survival.

Dec 6, 2023 · The produced GD2 scFv-IgG Fc antibodies, which are smaller in size than the intact antibody, could trigger the killing of GD2 expressed NB cell line SH-SY5Y by ADCC and ADCP mechanisms. The results indicate that chimeric scFv-hIgG Fc antibody, lacking heavy chain CH1 domain, could mediate antibody induced anti-tumor activities.

Jul 11, 2022 · Here, we show that reduced GD2 expression was significantly correlated with the mesenchymal cell state in neuroblastoma and that a forced adrenergic-to-mesenchymal transition (AMT) conferred...

Apr 14, 1994 · SH-SY5Y cells were found to express at least 12 different gangliosides. RA-induced neuronal differentiation led to a decrease in the content of GM2, GD3, and GD2 and to a 3-7 fold increased concentration of the ganglio-tetraosyl gangliosides GM1, …