To verify the protein expression from IVT mRNA in vitro, primary cultured fibroblast-like synoviocytes (FLS) and MH7A human synovial fibroblast cells were transfected with enhanced …

Mar 23, 2022 · To verify the protein expression of IVT mRNA in vitro, primary cultured human fibroblast-like synoviocytes (FLS) were transfected with enhanced green fluorescent protein …

FLS were transfected with in vitro-transcribed mRNA (IVT mRNA) and harvested at 24 h after transfection. a Western blotting of EGFP. b Images of FLS captured using fluorescence …

The mechanism of fibroblast-like synoviocyte (FLS) transformation into an inflammatory phenotype in rheumatoid arthritis (RA) is not fully understood. FLS interactions with invading …

The bait is localized to FLS by the fused C terminus of NS and visualized by the inherent fluorescence of the fused EGFP (Fig. 2c, left panels, middle and bottom rows).

In this study, rotational and translational diffusions of enhanced green fluorescent proteins (EGFPs) and EGFP tandem-oligomer were measured. We succeeded in the determination of …

Apr 3, 2025 · EGFP is expressed via an IRES to report cap-independent translation, while a subgenomic promoter (depicted with an angled arrow) enables transcription of an RNA …

We are developing retroviral-mediated gene transfer to human fibroblast-like synovial cells (FLS) as one approach to characterizing genetic pathways involved in synoviocyte pathophysiology. …

To verify the protein expression from IVT mRNA in vitro, primary cultured fibroblast-like synoviocytes (FLS) and MH7A human synovial fibroblast cells were transfected with enhanced …

Apr 1, 2002 · Centrifugal concentration of viral supernatant by 10- to 100-fold significantly increased the efficiency of viral transduction, with 50% or more of FLS expressing EGFP in …