In molecular biology, an exonic splicing enhancer (ESE) is a DNA sequence motif consisting of 6 bases within an exon that directs, or enhances, accurate splicing of heterogeneous nuclear RNA or pre-mRNA into messenger RNA .

Exonic splicing enhancers (ESEs) are cis-acting elements that activate the definition of an otherwise weak exon, promoting its inclusion into mature transcripts.

Feb 24, 2021 · In general, SR proteins play a positive role in splicing regulation and preferentially bind to exonic splicing enhancer (ESE) and intronic splicing enhancer (ISE).

Intronic Splicing Enhancers (ISEs) and Intronic Splicing Silencers (ISSs) are intronic cis -elements that play similar roles as ESEs and ESSs. Several bioinformatics tools to study or predict splice signals have been developed and are today available online.

Sep 16, 2012 · We observed a systematic overlap between ISEs and ESSs and established a general rule that all ISE motifs consistently inhibit splicing in exons. We further identified and analyzed putative trans...

Dec 17, 2004 · Exonic splicing silencers (ESSs) are cis -regulatory elements that inhibit the use of adjacent splice sites, often contributing to alternative splicing (AS). To systematically identify ESSs, an in vivo splicing reporter system was developed to screen a library of random decanucleotides. The screen yielded 141 ESS decamers, 133 of which were unique.

Mar 1, 2000 · Exonic splicing enhancers (ESEs) are discrete sequences within exons that promote both constitutive and regulated splicing. The precise mechanism by which ESEs facilitate the assembly of splicing complexes has been controversial. However, recent studies have provided insights into this question and have led to a new model for ESE function.

Jul 30, 2021 · A An ideogram shows splice-switching oligonucleotides (SSOs) targeting direct splicing site (5’SS or 3’SS), exon splicing enhancer (ESE) or inhibitor (ESI) and potential splicing sites. SSOs ...

EX-SKIP is simple utility that compares the ESE/ESS profile of a wild-type and a mutated allele to quickly determine which exonic variant has the highest chance to skip this exon. It calculates the total number of ESSs, ESEs and their ratio.

Splicing is regulated by cis-elements (ESE, ESS, ISS, and ISE) and trans-acting splicing factors (SR proteins, hnRNP, and unknown factors). The sequential phosphodiester transfer reactions involved in splicing are catalyzed by large ribonucleoprotein complexes known as spliceosomes.