A set of methods is described for channelrhodopsin-2 (ChR2) based synaptic circuit analysis that combines photostimulation of virally transfected presynaptic neurons’ axons with whole-cell …

First, the discovery that transfecting mammalian neurons with algae-derived light-sensitive ion channel channelrhodopsin-2 (ChR2), confers light-driven control over excitability of such …

Dec 15, 2010 · Postsynaptic depolarization induced by ChR2 can be combined with two-photon glutamate uncaging to potentiate visually identified dendritic spines. ChR2-mediated …

Dec 31, 2006 · We have combined millisecond activation of channelrhodopsin-2 (ChR2), a light-gated ion channel, with two-photon calcium imaging to investigate active synaptic contacts in …

Apr 15, 2007 · We show that the light-gated channel channelrhodopsin-2 (ChR2) is delivered to axons in pyramidal neurons in vivo. In brain slices from ChR2-expressing mice, …

Dec 23, 2022 · ChR2-eYFP was globally expressed in CaMKII+ pyramidal neurons of the AAV-injected mPFC (blue), whereas mPFC engram neurons expressed tdT (red).

Feb 2, 2009 · Here we developed an efficient Channelrhodopsin-2 (ChR2)-assisted method 6 - 8 to map the spatial distribution of synaptic inputs, defined by presynaptic ChR2 expression, …

Jun 1, 2016 · To investigate excitatory and inhibitory GABA actions in cortical neuronal networks, we present a novel optogenetic approach using a mouse knock-in line with conditional …

Feb 9, 2022 · For ChAT-ChR2 experiments, EPSC charge was calculated as the integral of the trace in a window of 65 ms starting with photostimulation. EPSC peak amplitude was …

Optogenetic stimulation of ChR2-positive CA2 fibers during patch-clamp recordings from CA1 pyramidal cells revealed an excitatory response (average EPSC amplitude −120±20 pA, …