Nov 6, 2022 · EGFP is a basic (constitutively fluorescent) green fluorescent protein published in 1996, derived from Aequorea victoria. It is reported to be a rapidly-maturing weak dimer with moderate acid sensitivity.

Fluorescence-activated cell sorting (FACS) is a flow cytometric method commonly used to isolate discrete populations of cells from heterogeneous cell pools. The technique of cell sorting derives from the principle that fluid streams in air are unstable and decay into spherical droplets as a consequence of reduction in free energy.

We developed a “comparative GPS profiling” strategy to identify proteins whose stabilities increased or decreased in response to stimuli. We used two pools of library cells, treated one with the stimulus of choice and sorted cells from each pool …

May 24, 2022 · Here we report a simple fluorescence activated cell sorting (FACS)-based enrichment method for direct assessment of efficacy and potential off-target effects of gene therapy co-delivered with an eGFP reporter to murine RPE cells using subretinal administration.

Jan 1, 2021 · Our data demonstrate that Sox9 EGFP levels provide readout of Yap activity and delineate BEC heterogeneity, providing a tool for assaying subpopulation-specific cellular function in the liver. We characterize expression of a Sox9 EGFP transgene in the mouse liver.

Mar 20, 2019 · We aimed to optimize a workflow to extract sufficient amounts of high-quality RNA from a limited number of FACS sorted cells from Tg (fli1a:GFP) zebrafish embryos, which can be used for accurate gene expression analysis.

FACS analysis of EGFP-expression in neurons infected with lentivirus vector. FACS analysis were taken at 2, 3, and 4 days after primary cerebral cortical neurons were infected by...

After 10 days, we harvested cells and sorted them by FACS (fluorescence‐activated cell sorting) to distinguish a population of cells expressing Sept2‐EGFP from wild type (wt) cells. We found that about 0.2% of cells exhibited a detectable expression of EGFP (Figure S2 ).

Jan 1, 2001 · To generate alternative reporter mice, an enhanced green fluorescent protein (EGFP) gene was targeted to the retroviral-trapped ROSA26 locus. Upon Cre-mediated excision of “Stop” sequences, EGFP was expressed ubiquitously during embryogenesis and in adult tissues (including T cells, B cells, and myeloid cells).

The cell-binding and internalization activity of EGFP-EGF were analyzed by fluorescence-activated cell sorting (FACS) and confocal microscopy. Results: EGFP-EGF protein was expressed in Escherichia coli and purified.