Lipoplex particle size determination by DLS of cholesterol mixtures was 106–118 nm, compared to 194–356 nm for lipoplexes prepared with the spermine conjugates only, and to 685 nm for the LinOS/DOPE 1:1 mixture.

Oct 3, 2014 · The objective of this study was to synthesize an environmentally friendly and simple methodology for the preparation of graphene using a recombinant enhanced green fluorescent protein (EGFP). The successful reduction of GO to graphene was confirmed using UV-vis spectroscopy, and FT-IR.

Apr 1, 2023 · The transfection efficiency of LNPs housing mRNA expressing eGFP was examined in human primary alveolar epithelial cells and lung fibroblasts, as well as A549 human alveolar basal epithelial cells and MRC-5 human fibroblasts, quantitatively using flow cytometry analysis and qualitatively via confocal microscopy. eGFP expression was evident in ...

Apr 11, 2025 · VectorBuilder's EGFP LNP-mRNA has been sequence optimized, fully QC'ed, and functionally validated. It can be used as a control for both in vivo and in vitro applications and can be ordered with or without modified nucleotide.

Oct 3, 2014 · The objective of this study was to synthesize an environmentally friendly and simple methodology for the preparation of graphene using a recombinant enhanced green fluorescent protein (EGFP). Results: The successful reduction of GO to graphene was confirmed using UV-vis spectroscopy, and FT-IR.

Jun 12, 2024 · Based on these observations, we have now investigated Arc oligomerization in vitro over a 1000-fold concentration range (~20 nM to ~20 μM) and performed single-point fluorescence fluctuation spectroscopy (FFS) measurements in live cells over a concentration range of ~200 nM to ~5 μM.

Nov 5, 2012 · The effects of varying the lipid formulation and changing the N/P charge ratio on the intracellular delivery of siRNA to HeLa cells and on the siRNA-mediated gene silencing of a stably expressed reporter gene (EGFP) were evaluated.

This study showed that EGFP-EGF1-conjugated PLGA nanoparticles could be effectively delivered to atherosclerotic plaques in vivo and taken up by VSMCs with high TF expressions in vitro.

Feb 5, 2025 · Heterogeneous nuclear ribonucleoprotein K (hnRNPK) is an RNA-binding protein containing low-complexity domains (LCDs), which are known to regulate protein behavior under stress conditions.

Sep 15, 2017 · This study reports the simultaneous delivery of EGFP siRNA and the chemotherapeutic drug, doxorubicin by means of the composition that results from the electrostatic interaction between positively charged siRNA-complexes of gold nanoparticles (AuNPs) capped with PEI, 25 kDa (P25-AuNPs) and negatively charged carboxymethyl cellulose formulated PL...