Epstein-Barr encoding region (EBER) in situ hybridization is the methodology of choice for the detection of the Epstein-Barr virus (EBV) in tissue sections. Because of the large numbers of copies of EBERs present in latently infected cells, non-isotopic methods can be used.

These quantitative methods will help to understand the pathogenesis of EBV-associated diseases and to manage patients with high viral loads. Currently, EBV-related neoplasia is accurately diagnosed by EBER-ISH (using biopsy samples) and EBV viral load tests (using blood samples).

Four hematopathologists independently interpreted EBV-encoded RNA (EBER) and latent membrane protein 1 (LMP1) histochemical stains from 40 cases of Hodgkin lymphoma and then reviewed the stains as a group to resolve discrepancies and to develop interpretation guidelines.

Detection of Epstein-Barr virus (EBV) may be achieved by various methods, including EBV-encoded RNA (EBER) in-situ hybridization (ISH) and immunohistochemistry (IHC) for latent membrane protein (LMP-1). We compared novel automated ISH and IHC techniques in pediatric lymphoproliferative disorders wit …

In Situ Hybridization (ISH) Formalin-fixed paraffin embedded (FFPE) tissue block with Corresponding H&E, and/or unstained slides sectioned from tissue block. Precut slides from paraffin blocks in 5 micron sections. 0.2 cm x 0.2 cm x 0.2 cm tissue. Minimum of 2 slides per antibod in 5 micron sections. Store specimen at room temperature.

Detection of Epstein-Barr virus (EBV)-encoded RNA in the diagnosis of EBV-associated conditions. For the initial technical component only in situ hybridization (ISH) stain performed, the appropriate bill only test ID will be reflexed and charged (ISTOI).

Jan 22, 2025 · This probe set labels all latent EBV-infected cells, including EBV-positive lymphoblastoid cell lines and EBV infected B-cell immunoblasts in infectious mononucleosis. It also reacts with EBV-associated undifferentiated nasopharyngeal carcinomas and with Reed-Sternberg cells in almost all EBV-associated Hodgkin lymphoma cases.

EBER stands for Epstein-Barr virus-encoded small RNAs. These are small, non-coding RNAs produced by the Epstein-Barr virus (EBV), a member of the herpesvirus family. EBERs are among the most abundant viral RNAs present in EBV-infected …

EBER ISH is widely accepted as a gold standard test to detect latent EBV infection in tissue sections (GULLEY, 2001; GULLEY and TANG, 2008). This method allows the identification of EBV in tissue sample by utilizing a specific probe which targets the EBER transcripts in latently infected cells of the tissue sections.

In situ hybridization (ISH) to Epstein-Barr virus (EBV)-encoded RNA (EBER) is considered the “gold standard” for detecting and localizing latent EBV in biopsy samples. Transcripts from the EBER1 and EBER2 genes are an appropriate target...