Mar 12, 2024 · Gabija is a highly widespread prokaryotic defence system that consists of two components, GajA and GajB8. GajA functions as a DNA endonuclease that is inactive in the …

Nov 22, 2023 · Gabija protein A (GajA) is a DNA endonuclease that tetramerizes to form the core of the anti-phage defence complex. Two sets of Gabija protein B (GajB) dimers dock at …

GajA was identified as a DNA nicking endonuclease but the functions of GajB and the complex remain unknown. Here, we show that synergy between GajA-mediated DNA cleavage and …

GajA assembles into a tetramer via interactions mediated by both the ATPase domain and the nuclease domain. We also revealed that the GajA and GajB assemble into a heteromeric …

Oct 28, 2023 · In this study, we report the results of structural and functional analyses of GajB protein from the Gabija anti-phage defense system in B. cereus VD045. The crystal structure …

In this work, we purify and characterize the Gabija complex (GajA and GajB) from B. cereus VD045 and present the structure of the Gabija complex at 3.6 Å resolution. The resolved …

May 1, 2023 · Gabija protein A (GajA) is a DNA endonuclease that tetramerizes to form the core of the anti-phage defense complex. Two sets of Gabija protein B (GajB) dimers dock at …

GajA is an OLD nucleases family pro-tein, while GajB belongs to the SF1 helicases. The Gabija complex has sequence-specific DNA nuclease activity and prefers circular rather than linear...

Here, to explore how the Gabija system is activated for anti-phage defence, we report its cryo-electron microscopy structures in five states, including apo GajA, GajA in complex with DNA, …

Gabija protein A (GajA) is a DNA endonuclease that tetramerizes to form the core of the anti-phage defence complex. Two sets of Gabija protein B (GajB) dimers dock at opposite sides of …