Jan 29, 2021 · PcG domains are marked by H3K27me3, which is deposited by the catalytic component of PRC2 complex, mainly Enhancer of Zeste Homolog 2 (EZH2) and sometimes EZH1 20. H3K27me3 marks are associated...

Feb 24, 2022 · Here, we report that in acute leukaemias, EZH2 has additional noncanonical functions by binding cMyc at non-PRC2 targets and uses a hidden transactivation domain (TAD) for (co)activator...

Dec 4, 2018 · To determine whether EZH2 is recruited to these exogenous DNA fragments, we performed EZH2 ChIP-qPCR using a forward primer that targets the plasmid backbone and a reverse primer that targets the inserted AR promoter fragment.

Enhancer of zeste homolog 2 (EZH2), a dynamic chromatin regulator in cancer, represents a potential therapeutic target showing early signs of promise in clinical trials. EZH2 ChIP sequencing data in 19 cell lines and RNA sequencing data in ten ...

Nov 27, 2024 · Here, we report that enhancer of zeste homolog 2 (EZH2) directly methylates poly (adenosine diphosphate–ribose) polymerase-1 (PARP-1), an essential enzyme involved in DNA repair, and regulates its activity.

Jul 31, 2019 · We show that EZH2 mostly works as a transcriptional inducer in ACC, through cooperation with the transcription factor E2F1 and identify three positive targets involved in cell cycle regulation...

Feb 1, 2017 · Our results showed EZH2 to be highly overexpressed in astrocytic tumors with a significant positive correlation with grade. Interestingly, ChIP-seq mapping revealed distinct differences in genes and pathways targeted by these …

Collectively, our results reveal crosstalk between H3K27me3 inhibition catalyzed by EZH2 and CpG island methylation mediated by DNMT1 within the wwc1 promoter, which synergistically silence wwc1 gene expression in TNBC.

Mar 20, 2017 · Enhancer of zeste homolog 2 (EZH 2) has been characterized as a critical oncogene and a promising drug target in human malignant tumors. The current EZH 2 inhibitors strongly suppress the enhanced enzymatic function of mutant EZH 2 in some lymphomas.

Mar 7, 2019 · Sequential native ChIP revealed that EZH2 co-occupies with R-loop chromatin, independent of the immunoprecipitation order (Figures 2 A and 2B). No DNA was recovered over β-actin, as expected, confirming that there was no detectable carryover from the first ChIP with the S9.6 antibody.