We now know that the MKI67 gene promoter itself is cell cycle-regulated, containing binding sites for the canonical G1-regulatory E2F family of transcription factors (Ishida et al. 2001), and that Ki-67 mRNA levels increase during G1 (Sobecki et al., 2016; Sobecki et al., 2017).

Sp1 regulates expression of genes that promote cell cycle progression, including E2F , which stimulates Ki-67 transcription . Upon passage of the G 1 restriction point, CDK4/CDK6 activation triggers phosphorylation of RB which releases E2F [31,32], resulting in …

Jul 31, 2018 · Ki67 staining is widely used as a proliferation indicator in the clinic, despite poor understanding of this protein’s function or dynamics. Here, we track Ki67 levels under endogenous control in single cells over time and find that Ki67 accumulation occurs only during S, G2, and M phases.

Jun 28, 2021 · We describe how expression of the MKI67 gene coding for Ki-67 is controlled during the cell cycle. MKI67 mRNA and Ki-67 protein are maximally expressed in G 2 phase and mitosis. Expression is...

We describe how expression of the MKI67 gene coding for Ki-67 is controlled during the cell cycle. MKI67 mRNA and Ki-67 protein are maximally expressed in G 2 phase and mitosis. Expression is dependent on two CHR elements and one CDE site in the MKI67 promoter.

May 14, 2017 · Here we show that cell-cycle regulation underlies variable Ki-67 expression in all situations analyzed, including nontransformed human cells, normal mouse intestinal epithelia and adenomas, human cancer cell lines with or without drug treatments, and …

CDK 4/6 complex initiate the phosphorylation of retinoblastoma protein (Rb) family in the G1 phase and release transcription factors associated with E2F. As Ki-67 protein is bound to the E2F proteins under cancer conditions upon E2F overexpression, Ki-67 mRNA accumulates.

Apr 1, 2019 · Studies carried out with breast tumour showed increased expression patterns of E2F (cell cycle transcriptional regulator), MCM2-MCM6 (replication-initiation complex proteins minichromosome maintenance 2–6) which is indicative of tumour proliferation state measured by Ki-67 labelling index.

Nuclear antigen Ki-67 is widely accepted as a cell proliferation marker in both research and can-cer diagnostic settings. Despite its extensive use and clinical value, very little is still known about the biological function of Ki-67. A recent work published in Cell Reports has revealed important novel aspects of Ki-67 regulation that could ...

Oct 1, 2018 · One level of regulation is transcriptional: the promoter of the gene encoding Ki-67 contains binding sites for the canonical G1-regulatory E2F family of transcription factors, leading Ki-67 mRNA levels to increase in G1 phase [8].