Aug 8, 2011 · Chromatin occupancy studies also show that activation of gene expression by GATA1 almost invariably involves cooccupation at the relevant genomic loci by TAL1/SCL, E2A proteins (E47 or E12), LMO2 and LDB1 (1, 2, 4, 5).

Feb 17, 2011 · Loss-of-function studies in mouse and zebrafish have revealed critical functions for LMO2 in the establishment of primitive and definitive hematopoiesis during embryonic development. 12,22,23 In normal blood development, the LMO2-LDB1 dimer mediates interaction between the heterodimer E2A/SCL and GATA-1 to form a multiprotein complex that ...

Jun 4, 2009 · The combination of ChIP-chip and transgenic assays suggested differential regulation of Lmo2 elements with Ets factors acting on the endothelial promoter, while an autoregulatory complex composed of Lmo2, Tal1, and Gata factors might activate distal hematopoietic elements.

The Ldb1, LMO2, SCL/E12 Multiprotein Complex Recognizes the SCL E-Box DNA-Binding Motif. The potential involvement of Ldb1 in binding the SCL consensus E-box motif was evaluated by gel shift analysis of nuclear extracts prepared from transfected cells.

Jan 10, 2013 · LMO2 seems to function primarily through forming transcription factor complexes with TAL1 or LYL1, GATA proteins, LDB1, and E12 or E47 to regulate gene expression.

GATA-binding protein 2 (GATA2) and LIM domain only 2 (Lmo2) form common transcription complexes during hematopoietic differentiation. Here we show that these two transcription factors also play a key role in endothelial cells (EC) and lymphatic EC (LEC) function.

Aug 1, 2005 · In the present study, we describe the temporal and spatial pattern of the expression of SCL, GATA-2 and Lmo2 during early neurogenic periods in the embryonic chick brain. The data demonstrate that the three transcription factors are not co …

Jan 1, 2001 · We now show that in erythroid cells Lmo2 forms a novel DNA-binding complex, with GATA-1, TAL1 and E2A, and the recently identified LIM-binding protein Ldb1/NLI. This oligomeric complex binds to a unique, bipartite DNA motif comprising an E-box, CAGGTG, followed approximately 9 bp downstream by a GATA site.

Nov 1, 2007 · They form a transcriptional complex: LMO2 has no direct evidence in DNA binding capacity but could act as a bridging molecule bringing together different DNA binding factors (TAL1, LDB1, E12/E47, GATA1) that are essential for hematopoiesis (e.g. in …

Aug 15, 2011 · Chromatin occupancy studies also show that activation of gene expression by GATA1 almost invariably involves cooccupation at the relevant genomic loci by TAL1/SCL, E2A proteins (E47 or E12), LMO2 and LDB1 (1, 2, 4, 5).