020080 STOCK Slc6a3 tm1(cre)Xz /J The DAT-cre "knock-in" allele has a nuclear-localized Cre recombinase inserted just upstream of the first coding ATG of the dopamine transporter gene ( Slc6a3 ; DAT).

These mice may be useful for studying gene function in dopaminergic neurons, such as drug addiction, Parkinson's disease and Attention Deficit-Hyperactivity Disorder (ADHD).<strong><br /></strong></p>

Oct 8, 2024 · DAT-Cre transgenic mouse lines have been particularly useful in resolving the diverse functions of the brain’s dopamine (DA) systems. Here we report DAT-Cre-driven reporter gene expression in cell bodies of non-dopaminergic limbic brain areas, including the lateral septum, the amygdala and the lateral habenula (LHb).

Oct 11, 2019 · For the DA system, we characterized the DAT-Cre 41, TH-Cre 42 and PITX3-Cre 43 mouse lines which express Cre under control of the dopamine transporter (DAT), tyrosine hydroxylase (TH), or...

Cre-mediated excision of the floxed region results in a knock-out of <em>Mcu</em> in dopaminergic neurons, useful in studies of Parkinson's disease (PD).

Feb 8, 2021 · Heterozygous mice that express Cre-recombinase under the dopamine transporter promoter (DAT-Cre knock in mice, or KI) are widely used for targeting midbrain dopamine neurons, under the...

Dat-Cre. 遗传背景：C57BL/6 . 品系类型：靶向突变 . 相关基因：Slc6a3. 应用领域： 使用 Dat-Cre 小鼠，科研工作者可特异性标记多巴胺能神经元，进而通过光遗传学、化学遗传学等操控手段以及钙成像、光电极等记录手段特异性探究单一脑区内多巴胺能神经元的功能。

Cre重组酶活性早在胚胎第15天就被观察到，并与内源性基因表达在成年多巴胺能细胞群(黑质(SN)和腹侧被盖区(VTA)，以及大脑后野)共定位。 在成人嗅球中检测到较低的Cre重组酶活性，类似于该组织中已知的较低的Slc6a3 (或DAT)表达。 虽然SN、VTA和纹状体DAT免疫染色的模式和强度在野生型和突变型小鼠中没有差异，但在杂合子中纹状体DAT蛋白水平中度降低(17%)，在纯合子中显著降低(47%)。 与野生型相比，纯合突变纹状体DAT蛋白水平的降低与神经肽PDyn ( …

Mar 15, 2024 · By taking advantage of DAT-Cre-tdTomato mice, we sought to deepen our understanding of DA neurons and their putative subtypes in the ENS. We conducted immunofluorescence staining in colonic tissues along with single-cell RNAseq and in situ hybridization to unravel the heterogeneity of DAergic neuronal populations.

Oct 31, 2019 · The study demonstrates that DAT-Cre transgenic mice, beyond their usefulness in recombination of floxed alleles in DA neurons, could be implemented as tools to achieve selective targeting in restricted excitatory and inhibitory neuronal populations within …