The direct antiglobulin test (DAT), also known as the Coombs test, is a crucial tool in diagnosing immune-mediated red blood cell (RBC) destruction (autoimmune hemolytic anemia).

Feb 1, 2017 · Studies using DAT results from healthy blood donors have found that up to 0.1% of healthy people have a positive DAT finding without evidence of hemolysis. 33 Of these, about two-thirds are positive with IgG and one-third, with complement.

The direct Coombs test, also referred to as the direct antiglobulin test (DAT), is used to detect if antibodies or complement system factors have bound to RBCs surface antigens. [5] The DAT is not required for pre-transfusion testing [6] but may be carried out by some laboratories.

May 11, 2022 · Direct Coombs test (sometimes called a direct antiglobulin test) looks at your red blood cells to see if there are antibodies attached to them. It’s often used to diagnose blood-related conditions such as autoimmune hemolytic anemia. People with autoimmune hemolytic anemia don’t have enough red blood cells because their body destroys them too soon.

Sep 12, 2022 · DAT testing typically involves using a polyspecific reagent consisting of IgG and complement C3. Indirect antiglobulin testing is clinically useful for the detection of circulating antibodies that have the potential to induce RBC hemolysis; this test is most commonly utilized for RBC phenotyping and in crossmatch screening for blood transfusion.

Sep 11, 2024 · The DAT is a serologic test to detect red blood cells (RBCs) that are coated with complement and/or antibodies in-vivo (in the body). Among physicians caring for patients at the bedside, this test is often referred to as the “Direct Coombs Test,” which makes we blood bank nerds nervous, but that’s ok!

In a patient with mixed-type autoimmune hemolytic anemia, the direct antiglobulin test commonly detects both IgG and C3. Paroxysmal cold hemoglobinuria is caused by a biphasic hemolysin, known...

Apr 4, 2012 · Fundamentally, the DAT is used to determine whether red blood cells (RBC) have surface bound immunoglobulin G (IgG) and/or complement. The main utility of the DAT is to categorize hemolysis as immune-dependent or immune-independent.

The ID-Cards containing standardized dilutions of an anti-IgG, anti-IgG1 and IgG3 subclasses, bring relevant clues in a context of positive DAT to better assess the risk of hemolysis.

The Direct Antiglobulin Test (DAT) is a hemagglutination test using reagent antibodies to human IgG and complement components. When the antiglobulin reagent is combined with washed RBCs, the Fc portion of the coating antibody molecules will bind to the reagent forming a bridge between adjacent RBCs to produce visible agglutination.