The GenPoint system locates target sites hybridized with biotinylated nucleic acid probes with high sensitivity and resolution. The kit includes stringent wash concentrate, peroxidase-conjugated streptavidin, biotinyl tyramide, and diaminobenzidine (DAB) chromogenic substrate.

Use Tyramide Signal Amplification (TSA) to amplify signal or detect low abundance proteins for IHC, spatial biology and IF applications.

Our Dako brand of high-quality diagnostic antibodies, reagents, instruments, software and expertise help hospitals and research labs make accurate tissue-based cancer diagnoses and determine the most effective treatment for cancer patients.

This chapter will briefly review both TSA and quantum dot IHC techniques, followed by examples of their use in my laboratory, and finally, provide useful trouble- shooting strategies and tips for improving TSA IHC detection.

When target expression is low or antibodies are low-affinity, a highly sensitive tyramide signal amplification (TSA) technique can be used, given that horseradish peroxidase (HRP)-conjugated secondary antibody can be introduced. It is very important to block any endogenous HRP activity.

Apr 1, 2018 · Here we determined if an automated diagnostic tissue stainer could be used for mIF incorporating tyramide signal amplification (TSA), and how the final image quality compared with sections stained semi-automatically or manually.

Mar 14, 2019 · Using the automated MTP, we demonstrated a 4-plex automated multistaining with clinically relevant biomarkers within 84 minutes, showing perfect agreement with the state-of-the-art microwave...

CSA (Catalyzed Signal Amplification) System を応用した in-situ hybridization 法用の超高感度検出キットです。 PCR 法に匹敵するほどの感度を持ち、シングルコピーの DNA も検出可能です。 PCR 法に必要な特別な装置や設備を必要とせず、また、熱による組織への影響やコンタミネーションによる偽陽性の問題もなく、より簡便で安定な結果を得ることができます。 本製品は研究用です。 その他の用途にご使用いただくことはできません。 本製品は研究用です。 その他の …

This procedure describes an automated system for staining multiplex immunofluorescence paraffin sections using the Bond RX by Leica Biosystems and its Research Detection System 2.

Jul 1, 2018 · Multiple fluorescent immunolabeling combined with TSA and elution with a striping buffer will be a valuable addition to the methods of visualizing the same species antibodies in both research and diagnostic praxis.