Sep 24, 2019 · Here, we report a PTEN-ARID4B-PI3K axis in which PTEN inhibits expression of ARID4B, while ARID4B is a transcriptional activator of the PI3K subunit genes PIK3CA and PIK3R2 that are crucial...

Prostate cancer cell lines and xenografts with known PTEN status (22RV1-PTEN +/+; DU145-PTEN +/−; PC3-PTEN −/−) and H1299 and HCT116 cancer cells were used to evaluate how PTEN loss affects RAD51 expression and PARPi sensitivity.

In SPRY2 KD DU145 cells, both PP2A inhibitor (okadaic acid) and GSK3B inhibitor (SB216763) treatments decreased nuclear PTEN localization and nuclear p-PTEN levels (Figure 8A and Supplemental Figure 7K).

The expression data cited previously suggest that PTEN plays a role in determining the clonogenic and tumorigenic potential of human PCaP cells. To test this hypothesis, PTEN levels were knocked down by validated shRNA1 and shRNA2 in …

Knockdown PTEN DU145 and 22Rv1 cells were augmented, and the stem cell-like properties were examined by cell viability and tumor sphere formation and treated by Akt IV inhibitor to provide the signal transduction pathway. Luciferase activity assays were performed.

Expression of PTEN mRNA and proteins were determined using RT-PCR and western blotting in RWPE-1 and DU145 cells. We also studied the role of PTEN in TGFβ effects on cell proliferation and migration in DU145 cells after transient silencing of endogenous PTEN.

PTEN overexpression in PC3 cells inhibited cell proliferation. Knockdown of endogenous PTEN enhanced cell migration in DU145 cells, whereas PTEN overexpression reduced migration in PC3 cells and reduced phosphorylation of AKT in response to TGF-β.

We show that the tyrosine kinase PTK6 (BRK) is a PTEN substrate. Phosphorylation of PTK6 tyrosine 342 (PY342) promotes activation, while phosphorylation of tyrosine 447 (PY447) regulates...

We describe the production of monoclonal antibody against recombinant human PTEN and the study of PTEN gene and protein expression in three commercially available human prostate cancer cell lines, PC-3, LNCaP, and DU 145.

Oct 3, 2022 · Here we find that HOIP, one important component of linear ubiquitin chain assembly complex (LUBAC), promotes prostate cancer (PCa) progression by enhancing AKT signaling in a PTEN-dependent...