Mar 20, 2021 · In this study, we compare different DNAse Hi-C protocols and identify the critical steps that significantly affect the efficiency of the protocol. In particular, we show that the SDS …

Sep 29, 2016 · Our method, termed DNase Hi-C, eliminates the reliance on restriction enzymes associated with Hi-C by digesting fixed chromatin with the endonuclease DNase I in the …

We recently demonstrated that the inactive murine X chromosome adopts a bipartite structure using a novel 3C protocol, termed in situ DNase Hi-C. Like traditional Hi-C protocols, in situ …

Mar 28, 2019 · DNase Hi-C is a method to detect and quantify pairwise interactions between chromosome regions across the entire genome. DNase Hi-C was designed to overcome the …

Jun 1, 2018 · We recently developed DNase Hi-C for mapping 3D genome organization, which uses DNase I for chromatin fragmentation. DNase Hi-C overcomes RE-related limitations …

We recently developed a Hi-C variant, DNase Hi-C, for characterizing 3D genome organization. DNase Hi-C employs DNase I for chromatin fragmentation, aiming to overcome restriction …

Mar 20, 2021 · We propose a new robust protocol for the preparation of DNAse Hi-C libraries from cultured human cells and blood samples supplemented with experimental controls and …

Dec 18, 2014 · Among other experiments, the team validated their approach in two human cell lines by coupling DNase Hi-C with a targeted DNA sequence capture technology to map fine …

In this study, we compare diferent DNAse Hi-C protocols and identify the critical steps that significantly afect the eficiency of the protocol. In particular, we show that the SDS quenching …

Our method, termed DNase Hi-C, eliminates the reliance on restriction enzymes associated with Hi-C by digesting fixed chromatin with the endonuclease DNase I in the presence of divalent …