In biology, a branched DNA assay is a signal amplification assay (as opposed to a target amplification assay) that is used to detect nucleic acid molecules. [1] A branched DNA assay begins with a dish or some other solid support (e.g., a plastic dipstick).

The branched-DNA assay uses the accumulation of branched enzyme-conjugated DNA probes on target DNA trapped on a solid phase. 30 Pre-amplifier and amplifier molecules are then hybridized to the captured target.

Sep 23, 2024 · These tests analyze variations in the sequence, structure, or expression of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA).

Providing a reliable means for direct quantification of viral load in clinical specimens, bDNA assays have been developed to measure hepatitis B virus DNA, hepatitis C virus RNA), human immunodeficiency virus type 1 RNA, and cytomegalovirus DNA.

The aim of this review is to inform biologists studying DNA methylation of the pros and cons of the different assays currently available; allowing them to make an informed choice when deciding the technique that would best suit their research needs.

DNA extraction is a method to purify DNA by using physical and/or chemical methods from a sample separating DNA from cell membranes, proteins, and other cellular components.

The branched DNA assay, hybrid capture assay, and Cleavase Invader assays are examples of signal amplification–based assays that have been used in quantitative assays for HIV and for detection of HPV, N gonorrhea and C trachomatis. 18, 19, 20

Achieve accurate and precise quantification of double-stranded DNA with Qubit dsDNA HS and BR Assay Kits. These dsDNA quantification kits enable quick and selective detection of low and high abundance DNA samples, and can distinguish …

InvitrogenTM gene expression assays based on branched DNA (bDNA) technology are versatile and enable a comprehensive approach to systems biology and translational sciences for verification and quantitation of biomarkers identified by next-generation sequencing or microarray studies, or from published literature.

The branched DNA (bDNA) assay provides a unique and powerful tool for reliable quantification of nucleic acid molecules. Fundamentally different from target amplification methods such as PCR, the bDNA assay directly measures nucleic acid molecules at physiological...