Jun 1, 2015 · The dorsomedial hypothalamus (DMH) integrates cutaneous thermosensory signals and regulates adaptive thermogenesis. Here, we study the function and synaptic connectivity of input from DMH cholinergic neurons to sympathetic premotor neurons in the raphe pallidus (Rpa).

Aug 15, 2023 · To verify whether LPB Vglut2 neurons directly innervate DMH neurons, we injected AAVs carrying Cre-dependent ChR2 into the LPB of Vglut2-Cre mice and recorded light-induced excitatory...

Oct 11, 2023 · In vivo Ca 2+ imaging reveals elevated DMH mWAKE activity during wakefulness and rapid eye movement (REM) sleep, while patch-clamp recordings show that DMH mWAKE neurons fire more frequently at...

Oct 16, 2024 · Here, authors demonstrate the role of PVH RUVBL2 in suppressing food intake and reveal two anorexigenic circuits of PVHRUVBL2 → ARCPOMC and PVHRUVBL2 → DMH.

Mar 7, 2018 · To express channelrhodopsin 2 (ChR2) in the POA-projecting neurons, we injected Cre-inducible rAAV2-retro-ChR2-eYFP into the POA of GAL-Cre mice and implanted an optic fiber into the DMH (Figures 4 A and S4 A).

Next, we examined whether activation of DMH GABAergic neurons is sufficient to induce torpor. To this end, we injected Vgat-cre mice unilaterally with AAV expressing Cre-dependent channelrhodopsin 2 (ChR2), or eYFP as control, in the DMH (Figure S4 A).

Jun 3, 2024 · In order to activate the excitatory neurons in the DMH, we expressed Channelrhodopsin-2 (ChR2) bilaterally in the DMH of vesicular glutamate transporter 2 (VGluT2) -Cre mice through injection of an adeno-associated virus (AAV) (Figure 2 D).

Jun 20, 2024 · We confirmed this synaptic connection by observing glutamatergic EPSCs evoked in DMH neurons by optogenetic activation of ChR2-expressing VMT axon terminals in acute brain slices (Fig. 5, A to C, n = 4 neurons).

Apr 11, 2015 · We found that DMH cholinergic neurons directly innervate sympathetic premotor neurons in the Rpa and that elevated cholinergic input to serotonergic neurons in the Rpa reduces BAT temperature through activation of M2 mAChRs.

Apr 11, 2015 · The dorsomedial hypothalamus (DMH) integrates cutaneous thermosensory signals and regulates adaptive thermogenesis. Here, we study the function and synaptic connectivity of input from DMH cholinergic neurons to sympathetic premotor neurons in …