DIF is useful in diagnosing cutaneous vasculitis and some other types of inflammatory skin disease: The optimal site for taking a biopsy of the skin depends on the suspected disease. …

Direct immunofluorescence is a vital diagnostic test for assessing vesiculobullous disorders, vasculitides, and connective tissue diseases. It is a robust and valuable technique that offers …

Direct immunofluorescence (DIF) test for tissue-bound autoantibodies provides a useful adjunct for the diagnosis of autoimmune bullous disorders, helping to classify histologically similar …

Direct SST employs patient's skin biopsy specimen, either freshly obtained or the one which was initially used for routine DIF. Indirect SST uses NHS as a substrate and is usually preferred …

BIOPSY: the edge of an active blister or perilesional erythematous or normal-appearing skin within 3-4 mm from a blister edge AVOID: specimens with detached epidermis, …

Biopsy specimens for DIF of immunobullous disease should be taken from nonbullous lesional skin or uninvolved perilesional skin within about 1 cm of a bulla (Fig 1). 3,4 Both bullous skin …

Aug 28, 2024 · A type of tissue biopsy called direct immunofluorescence (DIF) test is performed on skin or mucosal biopsy specimens and is useful in the diagnosis of many …

ABSTRACT: Direct immunofluorescence (DIF) is a valuable diagnostic tool in the dermatology clinic. The proper use of a biopsy for DIF is dependent on several factors, including …

Biopsy of a fresh lesion is recommended for DIF microscopy in connective tissue diseases and vasculitis. The appropriate sites of skin biopsy for DIF microscopy in various dermatological …

DIF results were positive in less than half of cases of hematoxylin and eosin biopsy-confirmed lupus erythematosus (23 of 47 [49%]). A total of 247 biopsies were submitted for clinical …