Dec 16, 2022 · On the other hand, as live-cell models, we use mouse fibroblasts expressing DDX4-YFP, which forms liquid-like nuage granules that are partitioned from chromatin 16, and different versions of...

Mar 5, 2015 · In order to follow the Ddx4 disordered regions within a cell, we generated a mimic, Ddx4 YFP, in which the DEAD-box helicase is substituted by YFP, a fluorescent protein of similar dimensions and overall charge to the helicase domain (Figure 1 A).

May 16, 2016 · The WT Ddx4 protein is a primary constituent of nuage membraneless organelles that occurs with a DEAD box helicase domain in place of the YFP moiety in Ddx4 YFP studied here.

Mar 5, 2015 · In order to follow the Ddx4 disordered regions within a cell, we generated a mimic, Ddx4YFP, in which the DEAD-box helicase is substituted by YFP, a fluorescent protein of similar dimensions and overall charge to the helicase domain (Figure 1A).

Sep 9, 2017 · A construct of Ddx4 retaining the disordered regions but substituting the DEAD-box helicase domain for YFP forms liquid membraneless organelles in nuclei when expressed in HeLa cells (15).

Apr 7, 2023 · The Ddx4 YFP mutant, which has a yellow fluorescent protein (YFP) instead of the central RNA helicase domain, also assembled the droplets, albeit in the nucleus.

Jan 1, 2017 · To verify that the LPPT-promoting potential of Ddx4 can be attributed to its disordered tails, a chimeric Ddx4 YFP protein was constructed, in which the DEAD-box helicase domain was substituted by a fluorescent protein YFP whose dimensions and charge were similar to those of the helicase domain.

Oct 20, 2024 · Adding competitively adsorbing 365 proteins, such as EGFP-NPM1, DDX4-YFP and GFP-G3BP1, was found to result in a reduction of 366 αSyn at the interface and the formation of multiphase condensates, providing a more favorable phase 367 for αSyn to partition into.

Jan 24, 2020 · To test the concept of noise reduction inside cells, we used a recombinant 2NT-DDX4 YFP protein that phase-separates in vitro (fig. S21). We expressed 2NT-DDX4 YFP inside HeLa cells and examined protein concentration and …

In order to follow the Ddx4 disordered regions within a cell, we generated a mimic, Ddx4 YFP, in which the DEAD-box helicase is substituted by YFP, a fluorescent protein of similar dimensions and overall charge to the helicase domain (Figure 1 A).