Jan 15, 2019 · To understand if the decreased AGR2 by MG132 is caused by protein stability, cycloheximide (CHX), a protein synthesis inhibitor, was included in the study below.

The whole cell lysate input was probed using anti-FLAG antibody to determine the expression of flag-ELL2. GAPDH was detected as loading control.

Jul 4, 2016 · By measuring the turnover of the well-characterized CMA substrate, GAPDH, following protein synthesis inhibition with cycloheximide (CHX) for 24h, we detected that …

Jun 12, 2023 · We found that GAPDH plays an important role in DSB repair and that GAPDH deficiency resulted in increased accumulation of DNA damage in cells. Immunofluorescence …

Jan 4, 2011 · Inhibition of new protein synthesis by cycloheximide (CHX) can block the survival pathway and, thus, is widely used in studying cell death. Indeed, treatment of primary MEF …

Oct 1, 2021 · CHX modulates mRNA expression of genes involved in early wound healing. Quantitative real-time PCR analysis of RAC1 (A), SERPINE1 (B) and TIMP1 (C) mRNA …

Jul 16, 1990 · GAPDH enzymatic activity is induced approx. sevenfold over resting levels. The induction of GAPDH mRNA is inhibited only slightly by CHX under conditions in which cell …

GAPDH served as a loading control (n = 3, *p < 0.01). Following the treatment of CHX, the Ca v 1.2 protein level showed a time-dependent decrease. The UBR6 reduction-induced increase in …

Dec 3, 2021 · Caspase-6 knockdown blocked p62 cleavage at D256 in response to TNFα + CHX treatment (see lane 6), while caspase-3 or caspase-9 knockdown did not reduce such p62 …

(A) Immunoblots for KLF4 and GAPDH in ES cells cultured with LIF/2i (undifferentiated) and 24 h after removal of LIF/2i, sampled at 0, 2, 4, 6, 8, and 12 h after cycloheximide (CHX) treatment. …