Channelrhodopsin-2 (ChR2), the first widely adopted optogenetic tool, is also a blue light activated cation channel. ChR2 is preferred over ChR1 because ChR2 has higher conductance at …

Sep 27, 2021 · The blue, green and red color emissions can be separately tuned by switching excitation wavelength to match respective spectral profiles of optogenetic proteins ChR2, …

ChETA：ChR2的突变体，使得神经元在激光刺激下可以发放200Hz的spike，而其他的ChR2 通道蛋白只能达到40Hz； 5. C1V1： 由ChR1及由团藻发现的VChR1组合在一起的通道蛋白，在 …

Nov 11, 2012 · We tested C1V1 T and C1V1 T/T in slice, given their improved frequency response compared with that of C1V1, and confirmed the potent responses to 1,040-nm light …

There are two versions of the ChR2 sequence, one containing the wildtype sequence and another containing condons optimized for mammalian expression (hChR2). In-frame fusions to …

Sep 1, 2013 · We engineered a variant of ChR, denoted red-activatable ChR (ReaChR), that is optimally excited with orange to red light (λ ∼ 590–630 nm) and offers improved membrane …

Oct 3, 2012 · Here, we establish the green-light activated ChR chimera C1V1 (from Chlamydomonas and Volvox ChR1′s) for use in C. elegans. We surveyed a number of red …

Sep 15, 2017 · In 2011, C1V1 enabled the first red light–driven spiking as well as in vivo combinatorial optogenetics [two populations separably controlled with red and blue light ], …

The C1V1 channel—comprised of Chlamydomonas and Volvox ChR1 domains and containing the E122T/E162T mutations that speed up the photocycle 15 —and ReaChR 20 possess an …

C1V1 T narrowly outperformed other members of the C1V1 family by this measure, consistent with high functional expression (Fig. 1b) and an approximately threefold-increased τ off relative …