Unlike other CDKs that require the binding of cyclins in order for their activation, CDK5 requires the binding of p35, p39, or p25 (a proteolytic fragment of p35) for activation. p35 (NCK5a, neuronal CDK5 activator) was first discovered due to its …

The neuronal-specific p35 pro-tein, which shares no homology to cyclins, was identi-fied by virtue of its association and activation of Cdk5. Gene targeting studies in mice have shown that the p35/Cdk5 kinase is required for the proper neuronal migration and …

Oct 1, 2001 · Association with its activators, p35 or p39, is necessary and sufficient for maximal activation of CDK5. CDK5 activity is dictated by the temporal and spatial expression and intracellular...

Nov 23, 2021 · p25 causes constitutive activation and mislocalization of Cdk5. p25 activates Cdk5 through direct binding, and p25 has an approximately 5- to 10-fold longer protein half-life compared to p35 [20], thereby prolonging Cdk5 activation.

Nov 6, 2019 · The atypical cyclin-dependent kinase 5 (CDK5) is considered as a neuron-specific kinase that plays important roles in many cellular functions including cell motility and survival. The activation of CDK5 is dependent on interaction with its activator p35, p39, or p25. These activators share a CDK5-bi …

Cyclin-dependent kinase 5 (Cdk5), which is activated by binding to p35 or p39, is involved in synaptic plasticity and affects learning and memory formation. In Cdk5 knockout (KO) mice and p35 KO mice, brain development is severely impaired because ...

Sep 11, 1998 · Here we show that p35, the activator of Cdk5, is a short-lived protein with a half-life (t1/2) of 20 to 30 min. Specific proteasome inhibitors such as lactacystin greatly stabilize p35 in vivo. Ubiquitination of p35 can be readily demonstrated in vitro and in vivo.

Dec 9, 1999 · To be activated, Cdk5 has to associate with its regulatory subunit, p35. We have found that p25, a truncated form of p35, accumulates in neurons in the brains of patients with Alzheimer's...

Under neurotoxic stress and neuronal injury conditions, CDK5R (p35) is cleaved by calpain to generate CDK5R1 (p25) in response to increased intracellular calcium. The elevated level of p25, when in complex with CDK5, leads to its subcellular misallocation as well as its hyperactivation.

The protein encoded by this gene (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form.