Plasmid pcDNA3-cdk5GFP from Dr. Li-Huei Tsai's lab contains the insert CDK5. This plasmid is available through Addgene.

Sep 20, 2018 · We found that activated CDK5 phosphorylated serine 108 in CLIC4, increasing CLIC4 protein stability, and accumulation. Pharmacological inhibition or shRNA-mediated silencing of CDK5 decreased...

We report here that Cdk5 nuclear localization relies on its binding to the cyclin-dependent kinase inhibitor p27. Cdk5 has no intrinsic nuclear localization signal; in the absence of p27, two weak nuclear export signals that bind CRM1 cause it to shuttle to the cytoplasm.

Here, we report a 12-amino acid long Cdk5-derived peptide that interferes with the Cdk5/p25 complex and ameliorates neurodegenerative phenotypes in cell and mouse models of Cdk5 hyperactivity. This small peptide is a promising candidate for a biotherapeutic against neurodegenerative diseases.

Aug 29, 2018 · CDK5 activation promotes ischemic neuronal death in stroke, with the recognized activation mechanism being calpain-dependent p35 cleavage to p25. Here we reported that CDK5-Tyr15...

Sep 4, 2024 · Here we demonstrate that CDK5 is active during mitosis and is necessary for maintaining mitotic fidelity. CDK5 is an atypical CDK owing to its high expression in post-mitotic neurons and...

Jul 11, 2014 · DN-CDK5-GFP, a dominant-negative CDK5 fused to GFP, was generated by single-nucleotide mutagenesis of D144 to N144 in CDK5-GFP. To produce YFP-hDrp1, we inserted the human Drp1 gene from pcDNA-hDrp1 9 into the pEYFP-C1 vector (Clontech).

Apr 4, 2010 · To study the effect of Cdk5 in different cellular compartments, we generated green fluorescent protein (GFP)-tagged expression constructs directing the kinase specifically in the nucleus (GFP-Cdk5-NLS), cytoplasm (GFP-Cdk5-NES), or in both compartments (GFP-Cdk5).

Jan 1, 2021 · Cdk5 and STAT3 were found in mitochondrial membranes of the injured sciatic nerve. Cdk5-GFP, which was translocated into the mitochondria by the mitochondrial target sequence (MTS), induced STAT3 phosphorylation in transfected DRG neurons and was sufficient to induce neurite outgrowth.

Oct 20, 2014 · We generated a set of GFP-tagged constructs for CDK5, GSK3β, and mitogen-activated protein kinase 1 (MAPK1/ERK2) and an additional set of p25 and p35 with a mCherry-tag.