Pan-cancer analysis showed that CD8A was highly associated with TMB, stemness and MSI among several tumor types including BCa (Supplementary Figure S1F–I). We further investigated the association between CD8A expression and IC50 values of …

Using a combination of Thymic Leukemia (TL) antigen tetramer, which directly binds CD8αα, anti-CD161, and anti-Vα7.2 antibodies we have been able for the first time to clearly define CD8αα expression on human CD8 T cells subsets.

Mar 13, 2024 · We find that cDC1 are required to generate broad CD8 responses against a range of diverse neoAgs. DC-therapy promotes immunogenicity of weaker neoAgs and strongly inhibits the growth of high...

Oct 10, 2021 · Exploratory analyses of patients with metastatic gastroesophageal cancer treated with NIVO+IPI, and patients with advanced hepatocellular carcinoma treated with NIVO, show a correlation between response and an inflammatory gene expression signature consisting of four genes [CD274 (PD-L1), CD8A, LAG3, and STAT1; refs.

Immune checkpoint blockade (ICB) therapy targeting cytotoxic T-lymphocyte-associated protein 4, pro-grammed death 1, and programmed death ligand 1 has shown durable remission and clinical success across different cancer types. However, patient …

Dec 14, 2021 · Here, a comprehensive immunogenomic analysis based on multi-omics data was performed to investigate the clinical significance and tumor heterogeneity between CD8 + T cell-related molecular clusters. We identified two distinct molecular clusters of ccRCC (C1 and C2) in TCGA and validated in E-MTAB-1980 cohorts.

The T cell reinvigoration correlated with response to pembrolizumab; however, this variable itself strongly correlated with the TMB, suggesting that an effective T cell response may not be enough to provide disease response if the TMB is too high [127].

Aug 11, 2022 · CD8A encodes the CD8 alpha chain of αβT cells, which has been proposed as a quantifiable indicator for the assessment of CD8 + cytotoxic T lymphocytes (CTLs) recruitment or activity and a robust biomarker for anti-PD-1/PD-L1 therapy responses.

Results: CD8A was identified as a hub gene in the DEG network and was found to be down-regulated in metastatic BC lesions. CD8A expression was highly correlated with the infiltration of CD8 + + T cells, and elevated CD8A expression was correlated with improved survival.

Mar 18, 2025 · The colorimetric reaction was performed using 100 µL of TMB substrate, incubating at 37 °C in the dark for 10–30 min, until a clear color gradient appeared in the diluted standard wells. ... Figure 2B shows the expression density of marker genes in the t-SNE space, with CD8A, GZMA, and NKG7 highly expressed in the upper right region, while ...