Then, CD44 and CD24 expression level in the three cell lines was further quantified using quantitative PCR (qPCR). Results showed that MDA-MB-231 and SUM159 cells have the high CD44 and low CD24 expression, while MCF7 cells have the opposite expression profile, i.e., a higher CD24 and lower CD44 expression (Fig. S1D).

May 3, 2022 · Cluster of differentiation 44 (CD44), a widely recognized marker for breast cancer stem cells (BCSCs), is extensively spliced into CD44 variant AS isoforms (CD44v) during the development of...

Jan 29, 2020 · Using single-cell transcriptome analysis and antibody screening, we identify CD44 as a marker of EHT enabling us to isolate robustly the different stages of EHT in the aorta-gonad-mesonephros...

Supplemental Figure 4: CD24/CD44 sorting strategy enriches for CD24− and CD44−positive populations. qPCR for CD24 (A) and CD44 (B) demonstrates population-specific enrichment for each marker, validating FACS parameters.

This reverse transcription-polymerase chain reaction (RT-PCR)-based method can be used to analyze the exon composition of each major CD44 mRNA species present in the cell.

Sep 29, 2011 · We used q-RT-PCR and exon-exon spanning assays to analyze the expression of four alternatively spliced CD44 isoforms as well as the total expression of CD44 in 187 breast tumors and 13 cell lines.

Sep 1, 2023 · CD44 cells were obtained first by transfecting human CD44 expression gene into the CHO–K1 cells, and the mRNA and protein expression of CD44 was confirmed using qPCR and flow cytometric assay.

Feb 20, 2013 · The CD44 high ESA low and CD44 high ESA high sub-populations are gated and show higher levels of staining of CD44 high ESA low cells for CD44. (B) QPCR analysis of CD44 variant gene expression in CD44 high ESA low cells relative to the CD44 high ESA high cells for the CA1, Met1 and Met2 lines shows marked differences in the isoform expression ...

CD44, a cell surface glycoprotein, is involved in many cellular processes including cell adhesion, migration and proliferation. However, whether and how the two molecules interact in breast...

Jan 4, 2022 · Real-time qPCR was performed to examine the gene expression activity. CD44 + OSCC CSCs can differentiate into adipocytes and adipogenesis in these cells decrease their proliferation and stemness gene expression. Adipogenic induction can make the cancer stem cells from OSCC tumors lose their stemness potential.