Difference from RNA-Seq and other gene expression analysis techniques. Different from Microarray and RNA-seq, CAGE is able to accurately identify transcriptional start sites (TSSs) and the corresponding promoter regions through sequencing the 3’ end of cDNA (5’ end of RNA).

CAGE measures RNA expression and maps TSS in promoters. In this method, RNA is first reverse-transcribed using random primers. The RNA cap and 3' ends are biotinylated. Nonhybridized, single-stranded RNAs are digested with RNase, leaving 5' complete cDNAs that are captured using streptavidin beads. The cDNA is processed for sequencing. Pros:

Cap analysis of gene expression (CAGE) is a gene expression technique used in molecular biology to produce a snapshot of the 5′ end of the messenger RNA population in a biological sample (the transcriptome).

CAGE-seq (Cap Analysis of Gene Expression AND deep Sequencing) 可以对mRNA中所有的TSS进行鉴定，这是通过加帽位点鉴定实现的（我更喜欢叫他“绿帽鉴定器”，咳咳）。 什么是加帽？ 在细胞核内的转录中，mRNA需要经历多种加工和修饰，包括剪接，5'端的加帽和3'端的加尾，以及碱基上的化学修饰等，才能变为成熟的mRNA，进入到细胞质进行翻译。 其中，加帽反应能够帮助细胞核的选择性孔道穿过，进入细胞质，还可以防止mRNA5'端的稳定性并增强翻译。 …

Jun 4, 2018 · A direct comparison of 5′-end RNA-seq methods reveals strong performance by CAGE, and identifies differential transcriptional start site usage among brain-related samples.

CAGE Preparation Kit is a ready-to-use product that includes reagents * and the protocol necessary for the preparation of a CAGE library, which allows the easy introduction of Cap Analysis of Gene Expression, CAGE, into your R&D.

Meet CAGE: the unique and powerful transcription starting site detection technology. Highly accurate and reliable way to discover Transcription Factor Binding Motifs, alternative promoters and new biomarkers by determining the cause of transcription.

Feb 23, 2012 · Cap-analysis gene expression (CAGE) provides accurate high-throughput measurement of RNA expression. CAGE allows mapping of all the initiation sites of both capped coding and noncoding...

Sep 1, 2021 · Cap analysis of gene expression (CAGE) was developed to detect the 5′ end of RNA. Trapping of the RNA 5′-cap structure enables the enrichment and selective sequencing of complete transcripts.

CAGE-seq can identify novel transcripts and alternative TSSs, providing insight into gene regulation and splicing events. Discover novel enhancer RNAs and alternative promoters for known genes to identify powerful biomarkers with clinical utility.