It mimics a fully processed mature mRNA. CleanCap ® Reagent AG produces a base-modified Cap-1 mRNA, which shows superior in vivo activity compared to Cap-0 mRNA produced by legacy capping methods such as mCap or anti-reverse cap analog (ARCA).

Cre recombinase is a widely used tool in the field of molecular biology. The enzyme's unique and specific recombination system is exploited to manipulate genes and chromosomes in a huge range of research, such as gene knock out or knock in studies.

Oct 13, 2023 · LNP variants deliver Cre mRNA in Ai9 mice. We subretinally delivered Cre-recombinase mRNA encapsulated LNP variants to test their ability to transfect retina in-vivo.

To more rapidly identify which types of cells an mRNA formulation transfects in vivo, we describe a Cre recombinase (Cre)-based system that permanently expresses fluorescent tdTomato protein in transfected cells of genetically modified mice.

RLPs deliver mature mRNA that leads directly to transient protein expression. There is no waiting for promoter-driven translation and there is no genomic integration. These VSV-G pseudotyped particles are capable of transferring RNA into dividing and nondividing cells.

Jan 29, 2023 · To evaluate functional Cre mRNA transfer through EVs, Ai9 reporter cells were incubated with three different doses of Cre EVs (2.2, 4.4 and 13.1 ×10 9 particles) for 72 hours and mRNA expression evaluated by digital droplet PCR (ddPCR).

The cre-loxP-mediated recombination system is an integral experimental tool for mammalian genetic and cell biology. This mRNA is capped with Cap1 structure with high capping efficiency. It has 100% substituted with N1-methyl-pseudoUridine for enhanced expression and reduced immunogenicity.

Cre recombinase is a tyrosine recombinase that catalyzes recombination between two loxP sites. The Cre mRNA is capped using m7G(5′)ppp(5′)A analog and polyadenylated to mimic a fully processed mature mRNA.

TriLink offers RUO and GMP custom CleanCap® Cap-1 mRNA production services in addition to our catalog mRNA offerings. Visit our Visit our website trilinkbiotech.com/mrna-cdmo-services or contact us at [email protected] for more information.

Apr 4, 2025 · The Cre-Lox system is a powerful tool in mouse genetics, enabling precise spatiotemporal control of gene expression and conditional knockout models. Since its development, it has transformed genome editing by facilitating targeted deletions, translocations, inversions, and complex modifications—double-floxed inverse orientation. Its utility extends beyond mice to rats, pigs, and zebrafish.