The Cre-lox system, derived from P1 bacteriophage, is a potent and specific system for controlling gene expression. The protein Cre recombinase recognizes 34 bp loxP sites, and the orientation and location of the loxP sites determines how the genetic material will be rearranged.

Cre（Cyclization Recombination Enzyme）是一种重组酶，来源于P1噬菌体，其基因编码区序列全长1029bp，为38kDa大小的、由343个氨基酸组成的多肽单体蛋白。 Cre重组酶的C-末端结构域包含催化活性位点，能够催化DNA分子中特定位点之间的重组，同时，Cre还能识别特异的DNA序列，即 loxP位点，使两个loxP位点间发生基因重组。 LoxP是Locus of X-overP1的缩写，是位于P1噬菌体中长度为34bp的一段序列，由两个13bp的反向回文序列和8bp的中间间隔序列共同 …

4 days ago · This regular plasmid gene expression vector system utilizes the LoxP-Stop-LoxP (LSL) cassette to achieve Cre-mediated conditional activation of gene expression in mammalian cells and animals. The LSL cassette comprises a LoxP-flanked (aka floxed) triple repeat of the SV40 polyadenylation sequence.

The Cre-lox system is a technology that can be used to induce site-specific recombination events. The system consists of two components derived from the P1 bacteriophage: the Cre recombinase and a loxP recognition site.

When bred to a strain expressing Cre recombinase under control of various tissue specific promoters, Cre recombination deletes the LSL cassette and allows the expression of the mutant KRAS oncogenic protein. This strain may be useful in studies of cancer and development.

To generate tumors sporadically in the lungs of K-ras LSL-G12D/+ mice, our laboratory initially used replication-deficient adenoviruses expressing Cre (Ad-Cre) to deliver transient Cre expression to infected cells of the lung 9, 10.

Its primary function is to disrupt bacterial cell walls by cleaving the β-1,4-glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine in bacterial peptidoglycan, leading to bacterial lysis.

Jun 22, 2016 · The modifed Ai targeting vector, Rosa-CAG-LSL-HA-DREADD-P2A-mCitrine, allows for robust transgene expression after cre-mediated excision of the upstream STOP sequence. The HA-tag allows for detailed spatial localization of the DREADD receptors after cre-excision via immunohistochemical staining.

常规质粒基因表达载体系统利用LoxP-Stop-LoxP（LSL）表达盒，在哺乳动物细胞和动物体内实现Cre介导的条件性基因激活表达。 LSL表达盒包含三个重复的SV40 polyA，序列两端为LoxP位点。

Jul 7, 2008 · Here, we generated a knockin mouse line termed LSL-R26Tva-lacZ with concomitant conditional expression of TVA and lacZ by targeting the Rosa26 locus. A loxP-flanked transcriptional stop cassette was used for conditional activation of TVA and LacZ expression in a Cre-recombinase-dependent manner.