These mutant mice may be useful in monitoring the Cre expression in living tissues, and tracing the lineage of such cells in embryos, young, and adult mice at desired time points.

These mice may have applications in studies requiring conditional gene manipulation in central nervous system microglia, as well as other Cx3cr1-expressing myeloid cell populations. Of note, The Jackson Laboratory also has available Cx3cr1 …

Mar 27, 2001 · Several Cre reporter strains of mice have been described, in which a lacZ gene is turned on in cells expressing Cre recombinase, as well as their daughter cells, following Cre-mediated excision of a loxP-flanked transcriptional "stop" sequence.

Feb 1, 2009 · In this study we introduce a new transgenic strain in which Cre recombinase is expressed exclusively from the IL-17F promoter. Crossing these mice to inducible ROSA26-enhanced yellow fluorescent protein (EYFP) reporter mice (9) allowed us to analyze the generation and location of Th17 cells.

Jun 1, 2014 · Here we report crossing thirteen different myeloid-Cre mouse strains to ROSA-EYFP reporter mice and assaying YFP expression in a variety of naïve unstimulated hematopoietic cells, in parallel. By focusing on myeloid subsets, we directly compare the relative efficiency and specificity of myeloid deletion in these strains under steady-state ...

Jan 1, 2009 · We show that cardiac perfusion of mice with a paraformaldehyde solution allows for the visualization of EYFP in situ in lymphoid tissues while maintaining staining of a variety of phenotypic markers and structural features.

6 days ago · Heterozygous Nestin-CreERT2 (courtesy of Ryoichiro Kageyama) or Dlx5/6-Cre mice (courtesy of Jiang-Fan Chen) were crossed with ROSA26-EYFP mice and genotyped with primers included in Table S1. Nuclear Cre activity in these mice was induced by daily intraperitoneal injections of tamoxifen dissolved in peanut oil, delivered at 125 mg/kg/day for 5 ...

Mar 21, 2025 · Prior to Cre recombination, the expression of ChR2_H134R/EYFP is blocked by an upstream LSL element. When crossed with Cre-expressing mice, the ChR2_H134R/EYFP fusion protein is specifically expressed in tissues where Cre is active in the offspring, making it an ideal tool for targeted optogenetic research.

Apr 4, 2025 · To further study the role of cholinergic pBF inputs to the CA1 region in fear extinction, we Cre-dependently expressed ChR2-EYFP (using AAV_EF1a::DIO-ChR2-EYFP) in pBF cholinergic neurons of ChAT-IRES-Cre mice (fig. S11, A and B) and found that optogenetic activation of cholinergic terminals in the CA1 region significantly enhanced fear ...

Apr 4, 2025 · The Cre-Lox system is a powerful tool in mouse genetics, enabling precise spatiotemporal control of gene expression and conditional knockout models. Since its development, it has transformed genome editing by facilitating targeted deletions, translocations, inversions, and complex modifications—double-floxed inverse orientation. Its utility extends beyond mice to rats, pigs, and zebrafish.